Portein kinase protocols 1st Edition By Alastair D Reith ISBN 1617371416 9781617371417

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ISBN 10: 1617371416 
ISBN 13: 9781617371417
Author: Alastair D Reith

Protein Kinase Protocols is a comprehensive and practical guide to the study and manipulation of protein kinases, one of the most critical families of enzymes involved in cellular signaling, regulation, and metabolism. Authored by renowned biochemist Alastair D. Reith, this first edition offers a wealth of detailed methodologies for researchers, students, and professionals working in biochemistry, molecular biology, and cellular physiology.

In this book, Reith presents a carefully curated collection of laboratory protocols that span a wide range of techniques essential for investigating protein kinase activity. From basic assays to advanced methods for structural and functional analysis, the protocols are designed to be accessible yet precise, ensuring that researchers of all levels can apply them with confidence in their own laboratories.

The book covers key topics such as:

• Protein Kinase Isolation and Purification: Detailed methods for isolating and purifying various types of protein kinases from diverse sources, including recombinant systems and native tissue preparations.
• Kinase Activity Assays: Step-by-step protocols for evaluating kinase activity using traditional radiolabeled assays as well as more modern, high-throughput techniques.
• Inhibitor Screening: Methods for testing the effects of small molecule inhibitors and peptides, crucial for drug discovery and therapeutic development.
• Kinase Substrate Identification: Techniques for determining substrates and understanding kinase specificity, essential for deciphering cellular signaling networks.
• Structural and Functional Analysis: Guidelines for characterizing protein kinases using advanced techniques such as mass spectrometry, X-ray crystallography, and NMR spectroscopy.

Reith also discusses the physiological roles of protein kinases in cell division, signal transduction, and disease mechanisms, particularly their involvement in cancer, neurodegenerative disorders, and metabolic diseases. Special attention is given to the interplay between kinases and other cellular processes, such as apoptosis, gene expression, and cell cycle regulation.

With an emphasis on reproducibility and precision, Protein Kinase Protocols is an indispensable resource for any laboratory focused on cellular signaling, drug discovery, or the molecular mechanisms underlying diseases. Whether you’re a beginner just learning the basics or an advanced researcher refining your techniques, this book provides the tools you need to advance your understanding of protein kinases and their roles in health and disease.

Portein kinase protocols 1st Edition Table of contents:

1. Introduction to Protein Kinases

   • 1.1 Overview of Protein Kinases and Their Role in Cellular Signaling
   • 1.2 Types of Protein Kinases: Serine/Threonine vs. Tyrosine Kinases
   • 1.3 Kinase Structure and Function
   • 1.4 Importance of Protein Kinases in Health and Disease

2. Protein Kinase Isolation and Purification

   • 2.1 Isolation of Protein Kinases from Cell Lysates
   • 2.2 Purification Techniques: Affinity Chromatography and Other Methods
   • 2.3 Expression and Purification of Recombinant Protein Kinases
   • 2.4 Quality Control of Purified Protein Kinases

3. Assaying Protein Kinase Activity

   • 3.1 Radiolabeled Assays for Measuring Kinase Activity
   • 3.2 Non-Radioactive Assays: Fluorescent and Colorimetric Methods
   • 3.3 High-Throughput Screening of Kinase Activity
   • 3.4 Kinetic Analysis of Kinase Reactions

4. Kinase Substrate Identification and Specificity

   • 4.1 Methods for Identifying Kinase Substrates
   • 4.2 Using Peptide Arrays to Study Substrate Specificity
   • 4.3 Mass Spectrometry-Based Approaches to Substrate Identification
   • 4.4 Kinase Consensus Motifs and Their Significance

5. Inhibitor Screening and Kinase Modulation

   • 5.1 Inhibitor Assays: Screening for Small Molecule Inhibitors
   • 5.2 Peptide and Antibody-Based Inhibitors
   • 5.3 Evaluating Kinase Inhibition in Cellular Systems
   • 5.4 The Role of Kinase Inhibition in Drug Discovery

6. Kinase Structural and Functional Analysis

   • 6.1 Structural Biology of Protein Kinases: X-ray Crystallography and NMR
   • 6.2 Determining Kinase Active Site and Allosteric Sites
   • 6.3 Cryo-Electron Microscopy in Kinase Research
   • 6.4 Functional Assays: Kinase Activation and Signal Transduction

7. Kinases in Cellular Signaling and Disease

   • 7.1 Role of Protein Kinases in Cell Cycle Regulation
   • 7.2 Kinase Pathways in Apoptosis and Survival
   • 7.3 Dysregulation of Kinases in Cancer
   • 7.4 Kinases and Neurodegenerative Diseases: Mechanisms and Therapies

8. Advanced Topics in Protein Kinase Research

   • 8.1 Targeted Kinase Inhibition in Therapeutic Development
   • 8.2 Kinase Profiling and the Development of Kinase Inhibitor Libraries
   • 8.3 Kinase Involvement in Metabolic Diseases and Drug Resistance
   • 8.4 Kinase Signaling in Stem Cells and Regenerative Medicine

9. Experimental Design and Data Interpretation

   • 9.1 Experimental Design for Kinase Studies
   • 9.2 Common Pitfalls and Troubleshooting
   • 9.3 Data Analysis: Quantifying Kinase Activity and Inhibition
   • 9.4 Statistical Methods in Kinase Research

10. Appendices

• 10.1 Recommended Buffers and Reagents for Kinase Assays
• 10.2 Protocols for Kinase Inhibitors and Substrate Libraries
• 10.3 Resources for Further Reading and Advanced Kinase Research
• 10.4 Glossary of Terms

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