Oral Biology Molecular Techniques and Applications Methods in Molecular Biology 2010th Edition by Gregory Seymour, Mary Cullinan, Nicholas Heng ISBN 1607618192 9781607618195

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ISBN 10:     1607618192  
ISBN 13: 978-1607618195
Author:         Gregory J. Seymour, Mary P. Cullinan, Nicholas C.K. Heng

Oral Biology: Molecular Techniques and Applications Methods in Molecular Biology 2010th Table of contents:

Gene Therapy of Salivary Diseases

• Overview of Gene Therapy Approaches for Salivary Glands
• Techniques and Strategies for Gene Delivery in Salivary Tissues
• Challenges and Future Directions

Collection, Storage, and Processing of Saliva Samples for Downstream Molecular Applications

• Best Practices for Saliva Collection
• Methods for Saliva Storage and Preservation
• Processing Saliva Samples for Molecular Analyses

Proteomic Analysis of Saliva: 2D Gel Electrophoresis, LC-MS/MS, and Western Blotting

• Techniques for Proteomic Profiling
• Applications in Saliva Analysis
• Interpretation of Proteomic Data

Transcriptomic Analyses of Saliva

• Methods for RNA Extraction from Saliva
• Gene Expression Analysis in Saliva
• Applications and Insights from Transcriptomic Studies

Oral Microbiology

The Oral Microbiota: General Overview, Taxonomy, and Nucleic Acid Techniques

• Overview of Oral Microbiota Composition
• Taxonomy of Oral Microorganisms
• Nucleic Acid-Based Techniques for Studying Oral Microbes

Microbial Community Profiling Using Terminal Restriction Fragment Length Polymorphism (T-RFLP) and Denaturing Gradient Gel Electrophoresis (DGGE)

• Methods for Profiling Oral Microbial Communities
• Applications and Limitations of T-RFLP and DGGE

Protocols to Study the Physiology of Oral Biofilms

• Techniques for Studying Biofilm Formation and Function
• Challenges in Oral Biofilm Research

Adhesion of Yeast and Bacteria to Oral Surfaces

• Mechanisms of Microbial Adhesion
• Factors Influencing Adhesion of Microbes to Oral Tissues

Quantitative Analysis of Periodontal Pathogens by ELISA and Real-Time Polymerase Chain Reaction

• ELISA for Detecting Periodontal Pathogens
• Real-Time PCR for Quantifying Pathogens

Bacterial Viability Determination in a Dentinal Tubule Infection Model by Confocal Laser Scanning Microscopy

• Techniques for Assessing Bacterial Viability in Dental Tissues
• Role of Confocal Microscopy in Infection Models

Characterization of Anti-competitor Activities Produced by Oral Bacteria

• Interactions Between Oral Bacteria
• Identification of Anti-competitor Substances

Natural Transformation of Oral Streptococci

• Mechanisms of Genetic Transformation in Oral Bacteria
• Role of Streptococci in Oral Health

Use of In Vivo-Induced Antigen Technology (IVIAT) to Identify Virulence Factors of Porphyromonas gingivalis

• IVIAT Technology in Microbial Pathogenesis
• Identifying Virulence Factors in Oral Pathogens

Oral Bacterial Genome Sequencing Using the High-Throughput Roche Genome Sequencer FLX System

• Advances in Oral Microbial Genomics
• Genome Sequencing Applications in Oral Bacteriology

Use of a Yeast-Based Membrane Protein Expression Technology to Overexpress Drug Resistance Efflux Pumps

• Techniques for Overexpressing Membrane Proteins
• Studying Drug Resistance in Oral Microorganisms

Cells and Tissues

Explant Culture of Embryonic Craniofacial Tissues: Analyzing Effects of Signaling Molecules on Gene Expression

• Culturing Embryonic Craniofacial Tissues
• Investigating Gene Expression Modulation

A Method to Isolate, Purify, and Characterize Human Periodontal Ligament Stem Cells

• Stem Cell Isolation from Periodontal Ligament
• Characterization and Potential Applications

Preclinical Methods for the Evaluation of Periodontal Regeneration In Vivo

• Animal Models for Periodontal Regeneration
• Assessing Regenerative Strategies

Proteomic Analysis of Dental Tissue Microsamples

• Methods for Microsampling of Dental Tissues
• Proteomic Profiling of Dental Tissues

Immunological Techniques: ELISA, Flow Cytometry, and Immunohistochemistry

• Overview of Immunological Techniques
• Applications in Dental Research and Diagnostics

Analysis of Immune Responses to Purified Recombinant Antigens of Periodontal Pathogens

• Immune Responses to Periodontal Bacteria
• Use of Recombinant Antigens for Immune Profiling

Single-Strand Conformation Polymorphism Analysis for the Diagnosis of T-Cell Clonality in Periodontal Disease

• SSCP Analysis for Diagnosing Clonal T-Cell Expansion
• Applications in Periodontal Disease Diagnosis

Real-Time PCR Focused-Gene Array Profiling of Gingival and Periodontal Ligament Fibroblasts

• Gene Expression Profiling in Gingival and Periodontal Cells
• Real-Time PCR as a Diagnostic Tool

The Use of Gene Arrays in Deciphering the Pathobiology of Periodontal Diseases

• Gene Array Techniques in Periodontal Research
• Understanding the Pathobiology of Periodontal Diseases

Bioinformatics Techniques in Microarray Research: Applied Microarray Data Analysis Using R and SAS Software

• Bioinformatics Approaches for Microarray Data
• Analysis Using R and SAS in Dental Research

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