Oral Biology Molecular Techniques and Applications 2nd Edition by Gregory Seymour, Mary P Cullinanr, Nicholas CK Heng ISBN 1493966839 9781493966837

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ISBN 10: 1493966839 
ISBN 13: 9781493966837
Author: Gregory Seymour, Mary P Cullinanr, Nicholas CK Heng

This second edition volume presents a selection of cellular and molecular techniques that can be adapted to cover a range of applications and diseases. The book is divided into three sections: saliva and oral diseases, molecular biosciences, and cell and tissues. The first section contains chapters that discuss proteomic analyses by mass spectrometry and NMR-based metabolomics that can be used to not only study saliva, but also to assess other oral fluids such as gingival crevicular fluid. The second section contains chapters that profile oral microbial communities, quantitative real-time PCR, and adhesion of yeast and bacteria to oral surfaces. The third section deals with a range of approaches that enable the behavior of cells and tissues in both health and disease to be analyzed at the molecular level. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Oral Biology: Molecular Techniques and Applications, Second Edition is a useful resource for oral biologists at all levels (students, early career researchers, and experienced veterans), and it provides a ready reference to new techniques and approaches that can be used to answer numerous specific scientific questions that may lead to a deeper understanding and treatments of oral diseases.

Oral Biology Molecular Techniques and Applications 2nd Table of contents:

Section 1: Introduction to Oral Biology

1. Overview of Oral Biology

   • Definition and Scope of Oral Biology
   • The Role of Molecular Biology in Oral Health
   • Historical Perspectives in Oral Biology

2. Basic Concepts in Molecular Biology

   • Structure and Function of DNA, RNA, and Proteins
   • Gene Expression and Regulation
   • Molecular Genetics in Oral Biology

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Section 2: Molecular Techniques in Oral Biology

3. Techniques in Molecular Biology

   • Polymerase Chain Reaction (PCR) and Applications
   • DNA Sequencing Methods
   • Gel Electrophoresis and Hybridization Techniques
   • CRISPR-Cas9: Gene Editing Applications in Oral Biology

4. Proteomics and Metabolomics

   • Introduction to Proteomics in Oral Health
   • Mass Spectrometry and Protein Identification
   • Metabolomics: Applications in Oral Biology

5. Microarray and Next-Generation Sequencing (NGS)

   • DNA Microarrays in Oral Health Research
   • RNA Sequencing and Transcriptomics
   • High-Throughput Genomic Technologies

6. Bioinformatics and Data Analysis

   • Analyzing Molecular Data: Tools and Software
   • Interpreting Big Data in Oral Biology Research
   • Databases and Resources for Oral Health Research

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Section 3: Molecular Pathogenesis of Oral Diseases

7. Oral Cancer: Molecular Mechanisms

   • Genetic Alterations in Oral Cancer
   • Biomarkers in Oral Cancer Diagnosis and Prognosis
   • Molecular Pathogenesis of Oral Squamous Cell Carcinoma

8. Periodontal Disease: Molecular Mechanisms

   • Genetic and Environmental Factors in Periodontal Disease
   • Role of Inflammation and Immune Response
   • Molecular Pathways in Periodontal Tissue Destruction

9. Caries and Tooth Development

   • Molecular Basis of Dental Caries
   • Enamel Formation and Mineralization
   • Genetic Factors in Tooth Development and Disease

10. Oral Infections and Immune Responses

    • Molecular Mechanisms in Oral Infections
    • Host Pathogen Interactions in Oral Diseases
    • Immune Response in Oral Infections

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Section 4: Molecular Applications in Oral Health

11. Molecular Diagnostics in Dentistry

    • Genetic Screening and Diagnostics in Oral Health
    • Advances in Biomarkers for Oral Diseases
    • Diagnostic Techniques in Oral Cancer

12. Molecular Therapeutics in Oral Health

    • Gene Therapy and Its Applications in Oral Diseases
    • Nanotechnology in Oral Biology and Medicine
    • Therapeutic Approaches for Periodontal and Dental Caries Treatment

13. Stem Cells and Regenerative Medicine in Dentistry

    • Stem Cells in Dental Tissue Regeneration
    • Role of Stem Cells in Periodontal and Oral Cavity Repair
    • Clinical Applications and Future Directions

14. Personalized Medicine and Oral Health

    • Pharmacogenomics in Dentistry
    • Tailored Treatments for Oral Diseases Based on Genetic Profiles
    • Future Perspectives in Precision Oral Medicine

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Section 5: Emerging Trends and Future Directions

15. Advances in Oral Microbiome Research

    • Role of Oral Microbiome in Health and Disease
    • Molecular Techniques to Study Oral Microbes
    • Microbiome Modulation for Oral Health Applications

16. Oral Cancer: Novel Molecular Therapies

    • Targeted Therapy in Oral Cancer
    • Immunotherapy in Oral Cancer Treatment
    • Emerging Molecular Markers for Early Detection and Prognosis

17. Regenerative Approaches in Oral Health

    • Tissue Engineering for Oral Regeneration
    • Molecular Mechanisms of Bone and Soft Tissue Repair
    • Clinical Applications in Oral and Maxillofacial Surgery

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Section 6: Conclusion

18. Future of Molecular Oral Biology
    • Integration of Molecular Biology with Clinical Dentistry
    • Challenges and Opportunities in Oral Health Research
    • Promising Areas for Future Molecular Research in Dentistry

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