Methods of Cell Separation 1st Edition by Sharpe 9780444809278

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ISBN 13: 9780444809278

Author: P.T. Sharpe

Methods of Cell Separation brings to the attention of researchers at all levels the variety of methods available for separating viable populations of cells. Methods are grouped into 3 categories based on the criteria of separation, namely; size or density; nonspecific surface properties; and specific surface properties. The principle of each method is described together with general and, where possible, specific protocols for conducting cell separation experiments. A central theme of the book is the separation of populations of blood lymphocytes which is used as an example for each method. Methods of Cell Separation is distinguished by three powerful assets: descriptions of the majority of cell separation methods currently being used; details of the experimental procedures involved in each method; and comparisons of the different methods for separating cell populations with particular reference to blood lymphocytes. An excellent addition to a distinguished series, and extremely useful as a laboratory manual.

Methods of Cell Separation 1st Table of contents:

Chapter 1. Introduction

Chapter 2. Methods of cell counting and assaying cell viability

2.1. Direct counting methods

2.2. Indirect cell counting methods

2.3. Cell viability

Chapter 3. Centrifugation

3.1. Introduction

3.2. Differential pelleting

3.3. Density gradient centrifugation

3.4. Density gradient cell separation

Chapter 4. Unit gravity sedimentation

4.1. Introduction

4.2. STAPUT apparatus

4.3. Other types of apparatus

Chapter 5. Centrifugal elutriation

5.1. Introduction

5.2. Beckman JE-6B elutriator system

5.3. Examples of cell separations

5.4. Separation of cells with different densities

Chapter 6. Aqueous two-phase partition

6.1. Introduction

6.2. Factors that influence partition

6.3. Cell partition

6.4. Single-step partition

6.5. Two-step partition

6.6. Multiple partition-thin layer countercurrent distribution

6.7. Fractionation of human peripheral blood lymphocytes with TLCCD

6.8. Separation of differentiating D. discoideum cells

6.9. Effect of protease treatment on cell partition

6.10. Computer analysis of TLCCD profiles

Chapter 7. Electrophoresis

7.1. Introduction

7.2. Free-flow electrophoresis

7.3. Biostream

7.4. Density gradient electrophoresis

Specific surface methods – an introduction to Chapters 8–13

Chapter 8. Affinity chromatography

8.1. lntroduction

8.2. Types of ligand interaction

8.3. Types of adsorbant

8.4. Sepharose 6MB macracrobeads

8.5. Affi-gel

8.6. lmmobilisation of ligands through cleavable bonds

8.7. Glass and plastic beads

Chapter 9. Affinity separation on fibres and surfaces

9.1. Introduction

9.2. Separation on unmodified fibres

9.3. Separation on modified fibres

9.4. Separation of unmodified surfaces

9.5. Separation on ligand coated surfaces

9.6. Separation on sephadex G-10

Chapter 10. Affinity partition

10.1. Introduction

10.2. Antibody affinity cell partition

Chapter 11. Flow sorting

11.1. Introduction

11.2. Principle

11.3. Signals produced

1.4. Cell separation

Chapter 12. Magnetic cell sorting

12.1. lntroduction

12.2. Types of apparatus

12.3. Preparation of magnetic microspheres

Chapter 13. Separation of cells in different phases of the cell cycle

13.1. Introduction

13.2. Centrifugation

13.3. Centrifugal elutriation

13.4. Unit gravity sedimentation

13.5. Phase partition

13.6. Flow sorting

13.7. Mitotic wash-off

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