Methods in Neurosciences 1st edition by Ian Phillips, Dwight Evans ISBN 0121852946 9780121852948

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ISBN 10:       0121852946
ISBN 13: 978-0121852948
Author:         M. Ian Phillips, Dwight Evans

Methods in Neurosciences 1st Table of contents:

Section 1: General Techniques

1. Chapter 1: Introduction to Neuroscience Techniques

   • Overview of Neuroscience Research Methodologies
   • Approaches to Studying the Nervous System
   • Ethical Considerations in Neuroscience Research

2. Chapter 2: Basic Microscopy Techniques

   • Light Microscopy
   • Electron Microscopy
   • Fluorescence Microscopy
   • Imaging at the Cellular and Subcellular Levels

3. Chapter 3: Histological and Immunohistochemical Methods

   • Tissue Preparation and Staining Techniques
   • Immunohistochemistry and Immunofluorescence
   • Detection of Neural Markers
   • Quantifying Expression in Brain Tissue

Section 2: Electrophysiological Techniques

4. Chapter 4: Electrophysiology of Neurons

   • The Action Potential and Its Measurement
   • Patch Clamp Techniques
   • Extracellular Recording Techniques
   • Single-unit and Multi-unit Recordings

5. Chapter 5: Electroencephalography (EEG) and Electrocorticography (ECoG)

   • Principles and Techniques of EEG
   • Clinical and Research Applications of EEG
   • ECoG Techniques and Their Use in Neurosurgery

6. Chapter 6: Intracellular Recording and Voltage Clamping

   • Techniques for Intracellular Recording
   • Measuring Membrane Potential and Ionic Currents
   • Applications in Synaptic Function and Plasticity

Section 3: Molecular and Genetic Methods

7. Chapter 7: Molecular Biology Techniques in Neuroscience

   • PCR and RT-PCR for Gene Expression Analysis
   • Cloning and Sequencing of Genes
   • CRISPR/Cas9 Technology in Neuroscience Research
   • Genomic and Transcriptomic Approaches

8. Chapter 8: Gene Expression Analysis in the Brain

   • RNA Sequencing and Microarrays
   • In situ Hybridization Techniques
   • Quantitative PCR for Specific Gene Expression

9. Chapter 9: Transgenic Models and Viral Vectors

   • Creation of Transgenic Mice and Other Organisms
   • Use of Viral Vectors for Gene Delivery
   • Techniques for Specific Neural Targeting

Section 4: Imaging Techniques

10. Chapter 10: In Vivo Imaging of the Brain

    • MRI, fMRI, and Diffusion Tensor Imaging (DTI)
    • Positron Emission Tomography (PET)
    • Near-Infrared Spectroscopy (NIRS)

11. Chapter 11: Functional Imaging Techniques

    • Measuring Brain Activity with fMRI
    • Electrophysiological Imaging Techniques
    • Optical Imaging in Neuroscience Research

12. Chapter 12: Two-Photon Microscopy and Optogenetics

    • Principles and Applications of Two-Photon Microscopy
    • Optogenetic Tools and Techniques
    • Combining Imaging and Manipulation of Neuronal Activity

Section 5: Behavioral and Cognitive Methods

13. Chapter 13: Behavioral Testing in Neuroscience

    • Animal Models of Behavior
    • Rodent Behavioral Assays: Maze Tests, Open Field Tests, etc.
    • Cognitive and Motor Function Tests

14. Chapter 14: Measuring Cognitive Function in Humans

    • Neuropsychological Testing
    • Functional Magnetic Resonance Imaging (fMRI) in Cognitive Neuroscience
    • Behavioral Assessment and Task Performance

Section 6: Neurochemical Methods

15. Chapter 15: Neurotransmitter Systems and Receptor Analysis

    • Methods for Studying Neurotransmitter Release and Receptor Function
    • Radioligand Binding Assays
    • High-Performance Liquid Chromatography (HPLC) and Mass Spectrometry

16. Chapter 16: Neurochemical Profiling and Metabolomics

    • Techniques for Profiling Metabolites in Neural Tissues
    • Gas Chromatography-Mass Spectrometry (GC-MS)
    • Metabolomics and Brain Function

Section 7: Neurological Disorder Research

17. Chapter 17: Models of Neurodegenerative Diseases

    • Animal Models of Alzheimer’s, Parkinson’s, and Huntington’s Diseases
    • Assessing Cognitive Decline and Neurodegeneration
    • Therapeutic Targets and Drug Development Models

18. Chapter 18: Stroke and Injury Models

    • Inducing and Assessing Cerebral Ischemia in Animal Models
    • Mechanisms of Brain Injury and Repair
    • Post-stroke Neuroplasticity and Rehabilitation Models

19. Chapter 19: Psychiatric Disorders and Neuroscience Research

    • Animal Models of Depression, Anxiety, and Schizophrenia
    • Cognitive and Behavioral Assessments in Psychiatric Research
    • Neurobiological Mechanisms of Psychiatric Disorders

Section 8: New Frontiers in Neuroscience Research

20. Chapter 20: Stem Cell and Regenerative Approaches in Neuroscience

    • Stem Cells and Their Potential in Neuroscience
    • Tissue Engineering for Brain Repair
    • Gene Editing and Regenerative Medicine in the Brain

21. Chapter 21: Brain-Computer Interfaces (BCI) and Neuroprosthetics

    • Technologies for Direct Brain Interaction
    • Applications in Neuroprosthetics and Rehabilitation
    • Ethical and Practical Challenges in BCI

Conclusion

22. Chapter 22: Future Directions in Neuroscience Research Methods
    • Advancing Techniques in Neuroscience
    • Integrating Technologies and New Methodologies
    • The Future of Brain Research and Treatment Approaches

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