Methods in Molecular Biology Volume 113 by DNA Repair Protocols 1st Edition by Daryl Eukaryoti ISBN 1617371963 9781617371967
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Authors:Daryl S. Henderson , Series:Forensic [2] , Tags:Forensic Medicine and Toxicology , Author sort:Henderson, Daryl S. , Languages:Languages:eng , Publisher:Humana Press , Comments:Comments:UV-A, UV-B, and UV-C: This terminology, which divides the ultraviolet(UV) spectrum into three wave bands, was first proposed in 1932 by the Americanspectroscopist William Coblentz and his colleagues to begin to addressthe problem of standardizing the measurement of UV radiation used in medicine(1,2). Each spectral band was defined “provisionally†and “approximatelyâ€by the absorption characteristics of specific glass filters as follows: UV-A,400–315 nm; UV-B, 315–280 nm; UV-C, <280 nm (1). Although based onphysical specifications, these definitions were influenced by knowledge ofother UV phenomenology, including biological effects and physical properties.For example, wavelengths in the UV-B band were known to have potenterythemic effects, and wavelengths below 290 nm were known to be absentfrom sunlight (2) (because they are absorbed by stratospheric ozone). Moreover,the germicidal effects of UV-C wavelengths (principally around 266nm) from artificial sources were well-recognized (3). Today, the spectral bandsimplied by these terms may be found to vary from Coblentz’s original definitions,depending on the discipline. Environmental photobiologists, for example,generally use the following definitions: UV-A, 400–320, UV-B, 320–290, andUV-C, 290–200 (4).Relative centrifugal forces: The g-forces listed in this book are calculatedfor the maximum radius unless stated otherwise. For microcentrifugessimilar to Eppendorf’s 5410 and 5415 C models, maximum rotational speed(14,000 rpm) corresponds to ~12,000g and ~16,000g, respectively
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ISBN 10: 1617371963
ISBN 13: 9781617371967
Author: Daryl Eukaryoti
Methods in Molecular Biology Volume 113 by DNA Repair Protocols 1st Edition Table of contents:
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Introduction to DNA Repair
- Overview of DNA Repair Mechanisms
- Importance of DNA Repair in Cellular Processes
- Classification of DNA Repair Pathways
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Techniques for Analyzing DNA Repair
- Methods for Assessing DNA Damage and Repair Efficiency
- Use of DNA Repair Markers
- Fluorescence Microscopy and Imaging of DNA Repair
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Base Excision Repair (BER)
- Protocols for Analyzing Base Excision Repair Pathways
- Enzyme Assays and Substrate Preparation
- Detection of BER Products
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Nucleotide Excision Repair (NER)
- Experimental Systems for Nucleotide Excision Repair Studies
- Purification of NER Enzymes
- Repair Assays for DNA Lesions
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Mismatch Repair (MMR)
- Mismatch Repair Mechanism Overview
- Protocols for MMR Assays
- MMR Gene Mutant Analysis
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Homologous Recombination (HR) and Non-Homologous End Joining (NHEJ)
- Protocols for Studying DNA Double-Strand Break Repair
- Recombinase Enzyme Assays
- NHEJ and HR Pathway Analysis
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Direct Repair of DNA Damage
- DNA Photolyases and Alkyltransferases
- Direct Repair Mechanisms in Cells
- In Vitro Assays for Direct DNA Repair
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DNA Repair in Eukaryotic Cells
- Model Systems for Studying DNA Repair
- Protocols for Repair in Yeast, Mammalian Cells, and Cell-Free Systems
- Cell Cycle Regulation of DNA Repair
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DNA Repair in Prokaryotic Cells
- Repair Mechanisms in Bacteria
- Genetic Tools for Studying Prokaryotic DNA Repair
- Mutagenesis and DNA Repair in Bacterial Systems
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Genotoxicity and Mutagenesis Assays
- Assessing the Impact of DNA Repair Defects
- DNA Repair in Cancer Research
- Protocols for Assessing Mutagenic Potential of Compounds
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Data Analysis and Troubleshooting
- Quantification of DNA Repair Efficiency
- Troubleshooting Common Protocol Problems
- Interpretation of Experimental Results
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Future Directions and Emerging Techniques
- Advances in DNA Repair Research
- CRISPR/Cas9 and DNA Repair Applications
- Next-Generation Sequencing in DNA Repair Studies
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