Laboratory Techniques in Biochemistry and Molecular Biology 1st editon by Fallon, Booth, Bell ISBN 0444808620 9780444808622
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Authors:A.Fallon , Series:Biochemistry [26] , Tags:Medical; Biochemistry and Pharmacology , Author sort:A.Fallon , Languages:Languages:eng , Published:Published:Feb 2008 , Publisher:Elsevier , Comments:This book is intended to familiarize biochemists with HPLC. Theoretical aspects of each mode of chromatography are discussed in chapters 1-9, providing an understanding of the various modes of chromatography which are now possible using commercially available columns, from reversed phase to affinity. Practical aspects and instrumentation are covered in chapter 10. The bulk of the book, which follows, presents examples and applications of each mode of chromatography in current biochemical practice.« less; « less
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ISBN 10: 0444808620
ISBN 13: 978-0444808622
Author: A. Fallon, R.F.G. Booth, L.D. Bell
Laboratory Techniques in Biochemistry and Molecular Biology 1st Table of contents:
Chapter 1: Introduction to Biochemistry and Molecular Biology
- 1.1 Overview of Biochemistry
- 1.2 Introduction to Molecular Biology
- 1.3 Importance of Laboratory Techniques
- 1.4 Basic Laboratory Safety Guidelines
Chapter 2: General Laboratory Techniques
- 2.1 Glassware and Equipment
- 2.2 Common Laboratory Reagents
- 2.3 pH Measurement and Adjustment
- 2.4 Centrifugation Techniques
- 2.5 Heating and Cooling Methods
Chapter 3: Protein Analysis
- 3.1 Protein Structure and Function
- 3.2 Protein Purification
- 3.3 Enzyme Assays
- 3.4 Quantification of Proteins
- 3.5 Protein Separation Techniques
- 3.5.1 Gel Electrophoresis
- 3.5.2 Chromatography Methods
Chapter 4: Nucleic Acid Analysis
- 4.1 DNA and RNA Structure
- 4.2 Extraction and Purification of Nucleic Acids
- 4.3 Quantification of Nucleic Acids
- 4.4 Gel Electrophoresis for Nucleic Acids
- 4.5 Polymerase Chain Reaction (PCR)
- 4.6 DNA Sequencing Methods
Chapter 5: Spectroscopic Methods in Biochemistry
- 5.1 Absorption Spectroscopy
- 5.2 Fluorescence Spectroscopy
- 5.3 Nuclear Magnetic Resonance (NMR)
- 5.4 Mass Spectrometry
- 5.5 Atomic Absorption and Emission Spectroscopy
Chapter 6: Immunological Techniques
- 6.1 Antigen-Antibody Interactions
- 6.2 Enzyme-Linked Immunosorbent Assay (ELISA)
- 6.3 Western Blotting
- 6.4 Immunoprecipitation
- 6.5 Flow Cytometry
Chapter 7: Cell Culture Techniques
- 7.1 Basics of Cell Culture
- 7.2 Maintenance and Propagation of Cell Lines
- 7.3 Transfection Techniques
- 7.4 Cell Viability and Proliferation Assays
Chapter 8: Chromatographic Techniques
- 8.1 Principles of Chromatography
- 8.2 Thin Layer Chromatography (TLC)
- 8.3 High-Performance Liquid Chromatography (HPLC)
- 8.4 Gas Chromatography (GC)
- 8.5 Ion Exchange Chromatography
Chapter 9: Electrophoretic Techniques
- 9.1 Principles of Electrophoresis
- 9.2 Agarose Gel Electrophoresis
- 9.3 Polyacrylamide Gel Electrophoresis (PAGE)
- 9.4 Two-Dimensional Electrophoresis
- 9.5 Capillary Electrophoresis
Chapter 10: Molecular Cloning and Recombinant DNA Technology
- 10.1 Introduction to Molecular Cloning
- 10.2 Vectors and Cloning Methods
- 10.3 Transformation and Transfection
- 10.4 Expression of Recombinant Proteins
- 10.5 CRISPR/Cas9 Technology
Chapter 11: Bioinformatics and Computational Biology
- 11.1 Introduction to Bioinformatics
- 11.2 Sequence Analysis
- 11.3 Structural Bioinformatics
- 11.4 Databases and Tools
- 11.5 Computational Approaches in Genomics
Chapter 12: Advanced Techniques in Biochemistry and Molecular Biology
- 12.1 Cryo-Electron Microscopy
- 12.2 Single-Molecule Analysis
- 12.3 High-Throughput Screening
- 12.4 Proteomics
- 12.5 Metabolomics
Chapter 13: Data Analysis and Interpretation
- 13.1 Statistical Methods in Biochemistry
- 13.2 Bioinformatics Tools for Data Analysis
- 13.3 Troubleshooting Experimental Problems
- 13.4 Presentation of Results
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