Laboratory Handbook of Medical Mycology 1st Edition by McGinnis Michael ISBN 0124828507 9780124828506

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ISBN 10: 0124828507
ISBN 13: 9780124828506
Author: McGinnis Michael

Laboratory Handbook of Medical Mycology summarizes the concepts dealing with the laboratory aspects of medical mycology. The publication first offers information on basic terminology and classification, laboratory safety, and clinical specimens. Discussions focus on tissue, abscess, blood, bone marrow, and urine specimens, biological hazards, disinfection and sterilization, grounding of electrical equipment, waste disposal, asexual and sexual reproduction, and vegetative growth. The text then takes a look at mold and yeast identification, including fermentation, temperature studies, asci and ascospores, zygomycetes, cycloheximide resistance, and sporulation and sterile isolates. The manuscript ponders on susceptibility testing and bioassay procedures, culture collection, and quality control. Topics include proficiency evaluations, media and equipment control, depositing unusual isolates in major culture collections, reconstituting lyophilized cultures, bioassay to determine drug levels in body fluids, and in vitro susceptibility testing. The publication is a dependable source of data for laboratory technologists, microbiologists, and mycologists engaged in safely isolating and accurately identifying fungi of medical importance.

Laboratory Handbook of Medical Mycology 1st Table of contents:

Chapter 1. Basic Terminology and Classification
Vegetative Growth
Asexual Reproduction
Sexual Reproduction
Classification
Selected References
Chapter 2. Laboratory Safety
General Recommendations
Disinfection and Sterilization
Biological Hazards
Fire Hazards
Chemical Hazards
Flammable Liquid Hazards
Waste Disposal
Safety Equipment
Gas Cylinder Storage, transport, and Use
Grounding of Electrical Equipment
Selected References
Chapter 3. Clinical Specimens
Part 1. General Information
Selected References
Part 2. Techniques
Abscess Specimens
Blood Specimens
Bone Marrow Specimens
Cerebrospinal Fluid
Fluids
Hair
Nails
Skin Scrapings (stratum Corneum)
Sputum
Tissue Specimens
Urine Specimens
Selected References
Part 3.direct Examination of Clinical Specimens
Giemsa Stain
Periodic Acid-schiff Stain
Potassium Hydroxide Preparation
Selected References
Chapter 4. Mould Identification
Part 1.concepts
Hughes’s Experimental Classification System
Barron’s Classification System
General References
Part 2.techniques
Colony Characteristics
Confirmation of Dimorphic Fungi
Cycloheximide Resistance
Forcibly Discharged Conidia or Spores
In Vitro Hair Test
Mixed Cultures
Nutritional Studies
Proteolytic Activity
Rice Grain Test
Slide Culture Technique
Sporulation and Sterile Isolates
Subculturing Isolates
Tease Mount
Temperature Studies
Urea Hydrolysis
Selected References
Part 3. Taxonomy
Ascomycetes
Coelomycetes
Hyphomycetes
Zygomycetes
General Identification References
References Cited
Chapter 5. Yeast Identification
Part 1. Basic Concepts
Selected References
Part 2. Techniques
Asci and Ascospores
Assimilation
Chlamydospore Development (dalmau Plate)
Demonstration of Capsules
Direct Mounts
Fermentation
Forcibly Discharged Conidia
Germ Tube Test
Mixed Cultures
Nitrate Utilization
Pigment Formation by Cryptococcus Neoformans
Temperature Studies
Urea Hydrolysis
Selected References
Part 3. Taxonomy
Ascomycetous Yeasts
Basidiomycetous Yeasts
Imperfect Yeasts
Characteristics of Medically Important Yeasts
References Cited
Chapter 6. Susceptibility Testing and Bioassay Procedures
In Vitro Susceptibility Testing
Bioassay to Determine Drug Levels in Body Fluids
Synergism Studies
Selected References
Chapter 7. Culture Collection
Culture Records
Receiving New Isolates
Reconstituting Lyophilized Cultures
Storage Techniques
Difficult-to-revive Cultures
Mites
Mailing Cultures
Depositing Unusual Isolates in Major Culture Collections
Killed Cultures for Study or Teaching
Selected References
Chapter 8. Quality Control
General Recommendations
Media Control
Equipment Control
Proficiency Evaluations
Selected References
Chapter 9. Synopsis of the Mycoses
Aspergillosis
Blastomycosis
Candidiasis
Chromoblastomycosis
Coccidioidomycosis
Cryptococcosis
Eye Infections
Hair,nail,and Skin
Histoplasmosis Capsulati
Histoplasmosis Duboisii
Lobomycosis
Mycetoma
Otomycosis
Paracoccidioidomycosis
Phaeohyphomycosis
Rhinosporidiosis
Sporotrichosis
Zygomycosis
Selected References
Chapter 10. Media and Reagents
Alphacel Agar for the Gymnoascaceae
Antibiotic Medium 3 for Amphotericin . Susceptibility Testing,1%agar
Antibiotic Medium 3 for Amphotericin . Susceptibility Testing,2%agar
Aspergillus Differential Medium
Brain Heart Infusion (bhi)agar
Buffered Yeast Nitrogen Base for Synergism Studies
Caffeic Acid Agar
Carbon Basal Assimilation Medium for Yeasts (auxanographic)
Carbon Disks for Yeasts
Carbon Sources,fermentation
Carbon Sources,wickerham Liquid Assimilation
Casitone Medium for Miconazole Susceptibility Testing,agar
Casitone Medium for Miconazole Susceptibility Testing,broth
Cereal Agar for Ascocarp Production in the Gymnoascaceae
Christensen’s Urea Agar or Broth
Converse Liquid Medium (levine Modification) for Coccidioides
Cornmeal Agar
Cottonseed Agar
Czapek-dox-solution Agar
Czapek-dox-solution Agar for Aspergillus
Fermentation Broth
Germ Tube Test Medium
Glucose-yeast Extract Medium for Coccidioides
Gram Stain Reagents,burke’s Modification
Hair Test,in Vitro
Hay Infusion Agar
Histoplasma Mould-to-yeast-form Conversion Medium
Hugh and Leifson Agar for Dermatophytes
Kelley’s Agar
Kleyn’s Acetate Agar for Ascospores
Lactophenol and Lactophenol Cotton Blue Mounting Media
Malt Extract Agar
Malt Extract (me)agar
Malt Extract Agar for Aspergillus
Martin’s Medium (modified)
M -3 Medium for Amphotericin . Susceptibility Testing,broth
Merthiolate for Serum
Mite Control
W-acetyl -l -cysteine (nalc)reagents
Nitrate Assimilation Medium
Nitrate Reagents
Nitrogen Assimilation Medium for Yeasts (auxanographic)
Nutrient Agars for Dermatophytes, Carbon Studies
Nutritional Agars for Trichophyton
Oatmeal Agar for the Gymnoascaceae
Peptone Disks for Yeasts
Periodic Acid-schiff Stain Reagents
Phosphate Buffer,0.07 M (M/15)
Polyvinyl Alcohol Mounting Medium
Potassium Hydroxide Solutions
Potassium Nitrate Disks for Yeasts
Potato Dextrose Agar
Potato Sucrose or Dextrose Agar for Fusarium
Rice Extract Agar
Rice Grains
Sabhiagar
Sabouraud Dextrose Agar
Sabouraud Dextrose Agar for Dermatophyte Maintenance
Sporulation
Sabouraud Dextrose Agar with Cycloheximide and Chloramphenicol,or Chloramphenicol Alone
Sabouraud Dextrose Broth
Sodium Carbonate for Blood Cultures
Soil Extract Agar
Starvation Medium for Yeasts
Triton X -100 for Blood Cultures
Varidase for Blood Cultures
V-8 Juice Agar,18%
V -8 Juice for Ascospores
Water Agar,2%
Water Agar for Susceptibility Testing,2%
Yeast Extract-malt Extract (ym)agar
Yeast Extract-phosphate Agar
Yeast Morphology Agar
Yeast Nitrogen Base,buffered for Synergism Studies
Yeast Nitrogen Base Medium for 5 -fluorocytosine Susceptibility Testing,broth
Glossary
Appendix A: Some Simple Symmetrical Shapes*
Appendix B: Commonly Encountered Synonyms and Other Obsolete Names
Appendix C: Reported Fungal Pathogens of Man

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