Epigenetic Methods in Neuroscience Research 1st Edition by Nina Karpova ISBN 1493927531 9781493927531

Epigenetic Methods in Neuroscience Research 1st Edition by Nina Karpova – Ebook PDF Instant Download/Delivery. 1493927531, 978-1493927531
Full download Epigenetic Methods in Neuroscience Research 1st Edition after payment

Product details:

ISBN 10:       1493927531
ISBN 13: 978-1493927531
Author:         Nina Karpova

This volume presents state-of-the-art methods for reliable detection of epigenetic changes in the nervous system. Epigenetic Methods in Neuroscience Research  guides readers through methods for the analyses of chromatin remodeling, transposable elements, non-coding RNAs, such as miRNAs, and circadian oscillations, including: analysis of DNA methylation in neuronal and glial cells or small tissue samples; sensitive method for quantification of alternative methylated forms of cytosines by liquid chromatography/mass spectrometry; affinity-based detection of modified cytosines by immunohistochemistry or methylated DNA immunoprecipitation; chromatin immunoprecipitation, or ChIP; miRNA high-throughput profiling and the in situ detection of miRNA subtle expression in the brain; analysis of genes with alternative 3’UTRs; and the cite-specific delivery of chromatin-modifying drugs. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.

Concise and easy-to-use, Epigenetic Methods in Neuroscience Research provides multidisciplinary epigenetic approach to study genome, and neural, plasticity that will help the reader to successfully address the challenges associated with neurodevelopmental, psychiatric, and neurodegenerative disorders.

Epigenetic Methods in Neuroscience Research 1st Table of contents:

• Epigenetics Overview:

  • Basic Biology to Chromatin Modifying Drugs: The study of how external factors affect gene expression without changing the DNA sequence itself, potentially through modifications of histones and DNA (e.g., DNA methylation, histone acetylation).
  • Potential Clinical Applications: Focus on how epigenetic therapies might be used in disease treatment, particularly neurological conditions such as Alzheimer’s, depression, or Parkinson’s disease.

• Brain Epigenome Analysis:

  • Guide to Epigenetic Methods: A detailed exploration of the various epigenetic tools and techniques used to study gene expression and modification in the brain.
  • Analysis of DNA Methylation by Bisulfite Reaction in Neural Cells: This method is used to analyze the methylation status of DNA in orexin-producing neurons, a critical component for understanding neuropeptide functions in the brain.
  • Simultaneous Analysis of DNA Methylation and mRNA Expression in Rodent Brain: Techniques that allow for the study of both epigenetic changes and their effects on gene expression in parallel, helping link molecular changes to functional outcomes in the brain.
  • Quantification of 5mC and 5hmC Levels Using HPLC/MS: This method focuses on quantifying global DNA methylation (5-methylcytosine, 5mC) and hydroxymethylation (5-hydroxymethylcytosine, 5hmC) levels, key modifications in epigenetics.
  • Methylated DNA Immunoprecipitation (MeDIP): A method for enriching and identifying methylated DNA sequences in the genome, important for studying how methylation influences gene activity in neurons.

• Cell Type-Specific DNA Methylation:

  • DNA Methylation Analysis in Neurons and Glia: A technique that distinguishes methylation patterns specific to neuronal or glial cells, helping to understand how these cell types may have different epigenetic regulation and functions in the brain.

• Detection and Analysis Techniques:

  • Immunohistochemical Detection of Oxidized Forms of 5-Methylcytosine: This technique is used to study DNA modifications, specifically the oxidation of 5-methylcytosine, in embryonic and adult brain tissue, which may influence gene expression.
  • Histone Modification Analysis in Neural Cells: Study of post-translational modifications of histones (such as acetylation, methylation), which influence chromatin structure and gene expression.
  • Site-Specific Delivery of Epigenetic Modulating Drugs: Techniques to deliver drugs that can modulate epigenetic markers specifically to certain brain regions, a step towards therapeutic interventions.
  • Transposable Element Expression: Investigates the plasticity of the genome through the study of transposons (mobile genetic elements), which can contribute to genome instability and gene expression changes.

• Synaptic Plasticity and Protein Synthesis:

  • Role of Local Protein Synthesis in Synaptic Plasticity: Local protein synthesis at synapses plays a key role in synaptic plasticity, which underlies learning and memory. Methods for analyzing this process are crucial for understanding neural function and disorders.
  • In Situ Detection of Neuron-Specific MicroRNAs: MicroRNAs are small RNA molecules that regulate gene expression. Their specific detection in the brain provides insights into their role in neuronal function and brain diseases.
  • High-Throughput miRNA Profiling: A method for profiling microRNAs in the rat brain to identify patterns of expression that could be linked to various neurological conditions.

• Metabolomics and Body Clock:

  • Metabolites as Clock Hands: The study of blood metabolites to estimate internal body time, focusing on how metabolic processes can influence or reflect circadian rhythms.

People also search for Epigenetic Methods in Neuroscience Research 1st:

epigenetics neuroscience
an epigenetic barrier sets the timing of human neuronal maturation
neuroscience epigenetics
dna methylation epigenetics
how epigenetics influences gene expression