Enzyme Stabilization and Immobilization Methods and Protocols 2nd Edition by Shelley Minteer ISBN 1493982192 9781493982196

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ISBN 10:       1493982192
ISBN 13: 978-1493982196
Author:         Shelley D. Minteer

This volume introduces the reader to the field of enzyme stabilization and the different theories of enzyme stabilization, including the use of immobilization as a stabilization technique. The first part of the book focuses on protocols for enzyme stabilization in solutions including liposome formation, micelle introduction, crosslinking, and additives. The second part of the book discusses protocols for enzyme stabilization during enzyme immobilization, including common techniques like sol-gel encapsulation, polymer encapsulation, and single enzyme nanoparticle formation. Protocols for a variety of enzymes are shown, but the enzymes are chosen as examples to show that these protocols can be used for both enzymes of biological importance, as well as enzymes of industrial importance. The final part details spectroscopic protocols, methods, and assays for studying the effectiveness of the enzyme stabilization and immobilization strategies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Enzyme Stabilization and Immobilization: Methods and Protocols, Second Edition provides molecular biologists, biochemists, and biomedical and biochemical engineers with the state-of-the-art technical information required to effectively stabilize their enzyme of interest in a variety of environments (i.e., harsh temperature, pH, or solvent conditions).

Enzyme Stabilization and Immobilization: Methods and Protocols 2nd Table of contents:

• Introduction to the Field of Enzyme Immobilization and Stabilization

  • Overview of Enzyme Immobilization
  • Applications and Importance in Biotechnology

• Stabilization of Enzymes through Encapsulation in Liposomes

  • Mechanism of Liposome Encapsulation
  • Advantages and Challenges
  • Applications in Enzyme Stabilization

• Micellar Enzymology for Thermal, pH, and Solvent Stability

  • Use of Micelles for Enzyme Stabilization
  • Improving Thermal, pH, and Solvent Resistance
  • Mechanisms and Applications in Biotechnology

• Lipase Activation and Stabilization in Room Temperature Ionic Liquids

  • Properties of Ionic Liquids
  • Enzyme Stabilization and Activation
  • Applications of Lipase in Ionic Liquids

• Nanoporous Silica Glass for the Immobilization of Interactive Enzyme Systems

  • Structure and Properties of Nanoporous Silica
  • Immobilization Mechanisms
  • Benefits in Enzyme Applications

• Enzyme Stabilization and Immobilization by Sol-Gel Entrapment

  • Sol-Gel Process Overview
  • Mechanism of Entrapment
  • Applications in Enzyme Stabilization

• Nanoporous Gold for Enzyme Immobilization

  • Properties of Nanoporous Gold
  • Immobilization Methods
  • Enzyme Activity and Stability Enhancements

• Enzyme Stabilization via Bio-Templated Silicification Reactions

  • Bio-Templated Silicification Methods
  • Mechanisms for Enzyme Stabilization
  • Applications and Advantages

• The Immobilization of Enzyme on Eupergit Supports by Covalent Attachment

  • Properties of Eupergit Supports
  • Covalent Attachment Strategies
  • Applications in Industrial and Biomedical Settings

• Micellar Polymer Encapsulation of Enzymes

  • Encapsulation in Polymer Micelles
  • Stability and Protection of Enzymes
  • Practical Applications in Enzyme Technology

• Enzyme Stabilization via Cross-Linked Enzyme Aggregates

  • Process of Cross-Linking Enzyme Aggregates
  • Benefits for Stability and Activity
  • Applications in Industrial Enzyme Use

• Enzyme Immobilization in Polyelectrolyte Microcapsules

  • Polyelectrolyte Microcapsules for Immobilization
  • Mechanisms and Advantages
  • Industrial and Biomedical Applications

• Macroporous Poly(GMA-co-EGDMA) for Enzyme Stabilization

  • Overview of Poly(GMA-co-EGDMA) Material
  • Immobilization and Stabilization Methods
  • Applications in Enzyme Technology

• Enzyme-Nanoparticle Conjugates for Biomedical Applications

  • Role of Nanoparticles in Enzyme Immobilization
  • Benefits in Biomedical Applications
  • Examples of Enzyme-Nanoparticle Conjugates

• Enzyme Nanoparticle Fabrication

  • Methods for Fabricating Enzyme-Loaded Nanoparticles
  • Stability and Activity Considerations
  • Applications in Drug Delivery and Diagnostics

• Magnetic Nanoparticle Synthesis and Enzyme Immobilization

  • Synthesis of Magnetic Nanoparticles
  • Methods for Enzyme Immobilization on Magnetic Particles
  • Applications in Magnetic Separation and Biotechnology

• Cytochrome C Stabilization and Immobilization in Aerogels

  • Aerogel as a Stabilization Matrix
  • Stabilization of Cytochrome C
  • Applications in Biotechnology and Medicine

• Kinetic Measurement for Enzyme Immobilization

  • Methods for Measuring Kinetics of Immobilized Enzymes
  • Impact of Immobilization on Enzyme Activity
  • Tools for Kinetic Analysis in Enzyme Engineering

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