Environmental Microbiology A Laboratory Manual 1st Edition by Ian Pepper, Charles Gerba ISBN 0125506554 9780125506557

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ISBN 10: 0125506554
ISBN 13: 9780125506557
Author: Ian Pepper, Charles Gerba

Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental Biotechnology.
The first section includes experiments on soil as a habitat for microorganisms, and introduces the main types of soil microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays of microbial pathogens — bacteria, viruses, and protozoan parasites — used in food and water quality control as well as an exercise in applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction (PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify a bacterium useful in bioremediation.
Designed for maximum versatility and ease of use for both the student and instructor, each experiment is self-contained and includes theoretical, practical, and pedagogical material.

Environmental Microbiology A Laboratory Manual 1st Edition Table of contents:

Chapter 1: Introduction to Microbial Ecology

• Microorganisms and Their Role in the Environment
• Microbial Classification and Diversity
• Microbial Habitats and Niches

Chapter 2: Safety in the Laboratory

• Laboratory Safety Guidelines
• Personal Protective Equipment (PPE)
• Waste Disposal and Sterilization Methods
• Handling Infectious Agents

Chapter 3: Basic Laboratory Techniques

• Aseptic Techniques
• Microscopy and Staining Methods
• Media Preparation
• Sterilization and Disinfection

Chapter 4: Isolation and Enumeration of Environmental Microorganisms

• Dilution Plating and Spread Plating
• Colony Counting Techniques
• Selective Media for Environmental Samples
• Molecular Methods for Microbial Detection

Chapter 5: Water Quality and Microbial Analysis

• Waterborne Pathogens
• Standard Methods for Water Microbiology
• Coliforms and E. coli in Water Testing
• Detection of Protozoa, Viruses, and Algae in Water
• Indicator Organisms and Their Role in Water Quality

Chapter 6: Soil Microbiology

• Soil Microorganisms and Their Functions
• Techniques for Soil Sampling and Analysis
• Isolation of Soil Microorganisms
• Role of Soil Microbes in Nutrient Cycling

Chapter 7: Air Quality and Microbial Sampling

• Airborne Pathogens and Allergens
• Techniques for Air Sampling
• Impacts of Microbial Contaminants in Indoor Environments
• Fungal Spores and Bacterial Aerosols

Chapter 8: Wastewater and Sewage Treatment Microbiology

• Microbial Roles in Wastewater Treatment
• Aerobic and Anaerobic Treatment Systems
• Bioremediation and Biodegradation in Wastewater
• Environmental Impact of Wastewater Treatment

Chapter 9: Environmental Microbial Communities

• Biofilms in the Environment
• Symbiotic Relationships Between Microorganisms
• Microbial Succession and Ecological Interactions
• Methods for Studying Microbial Communities

Chapter 10: Environmental Health Microbiology

• Pathogens in Food and Water
• The Role of Microorganisms in Disease Transmission
• Risk Assessment and Control of Environmental Hazards
• Emerging Pathogens and Environmental Microbiology

Chapter 11: Molecular Techniques in Environmental Microbiology

• PCR and Gel Electrophoresis in Microbial Detection
• Metagenomics and Environmental DNA
• Flow Cytometry in Microbial Ecology
• Advanced Molecular Techniques for Environmental Sampling

Chapter 12: Case Studies in Environmental Microbiology

• Studies of Contaminated Water Sources
• Microbial Risk Assessment in Agricultural Environments
• Soil Contamination and Remediation
• Pathogen Monitoring in the Environment

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