Dental Stem Cells Regenerative Potential 1st Edition by Barbara Zavan, Eriberto Bressan ISBN 3319332996 9783319332994

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ISBN 10: 3319332996 
ISBN 13: 9783319332994
Author: Barbara Zavan, Eriberto Bressan

This book focuses on the basic aspects of dental stem cells (DSCs) as well as their clinical applications in tissue engineering and regenerative medicine. It opens with a discussion of classification, protocols, and properties of DSCs and proceeds to explore DSCs within the contexts of cryopreservation; epigenetics; pulp, periodontal, tooth, bone, and corneal stroma regeneration; neuronal properties, mesenchymal stem cells and biomaterials; and as sources of hepatocytes for liver disease treatment. The fifteen expertly authored chapters comprehensively examine possible applications of DSCs and provide invaluable insights into mechanisms of growth and differentiation. Dental Stem Cells: Regenerative Potential draws from a wealth of international perspectives and is an essential addition to the developing literature on dental stem cells. This installment of Springer’s Stem Cell Biology and Regenerative Medicine series is indispensable for biomedical researchers interested in bioengineering, dentistry, tissue engineering, regenerative medicine, cell biology and oncology.

Dental Stem Cells Regenerative Potential 1st Table of contents:

Dental Stem Cells (DSCs): Classification and Properties
Embryonic Origin and Components of the Tooth
Dental Stem Cells (DSCs)
Classification and Properties of DSCs
Dental Pulp Stem Cells (DPSCs)
DPSCs Identification, Isolation, and Characterization
Differentiation Potential of DPSCs
Stem cells from Human Exfoliated Deciduous teeth (SHED)
SHED Identification, Isolation, and Characterization
Differentiation Potential of SHED
Periodontal Ligament Stem Cells (PDLSCs)
PDLSCs Identification, Isolation, and Characterization
Differentiation Potential of PDLSCs
Dental Follicle Progenitor Cells (DFPCs)
DFPCs Identification, Isolation, and Characterization
Differentiation Potential of DFPCs
Stem Cells from Apical Papilla (SCAP)
SCAP Identification, Isolation, and Characterization
Differentiation Potential of SCAP
Gingival Mesenchymal Stem Cells (GMSCs)
GMSCs Identification, Isolation, and Characterization
Differentiation Potential of GMSCs
Natal Dental Pulp Stem Cells (NDP-SCs)
NDP-SCs Identification, Isolation, and Characterization
Differentiation Potential of NDP-SCs
Conclusions
References
Protocols for Dental-Related Stem Cells Isolation, Amplification and Differentiation
Election of Research Material
Dental-Related Tissues’ Genesis
Histogenesis
Organogenesis/Odontogenesis
Dental-Related Tissues’ Stem. Cell Lineages Differentiation Potential and Anatomic Location
Tooth Organ-Stem Cell Lineages
Immature Tooth Organ-Stem Cell Lineages
Dental Papilla Stem Cells: iTDPSC
Apical Papilla Stem Cells/Immature Root Papilla Stem Cells: SCAP/iRPSCs
Mature Tooth Organ-Stem Cell Lineages
Dental Pulp
Predeciduous Dentition Dental Pulp Stem Cells/Natal, Neo-Natal Dental Pulp Stem Cells NDP-SCs
Primary Dentition Dental Pulp Stem Cells: SHED
Secondary Dentition Dental Pulp Stem Cells: DPSCs
Tooth-Supportive Tissues-Stem Cell Lineages
Immature Tooth-Supportive Tissues-Stem Cell Lineages
Dental Follicle Stem/Progenitor Cells: DFSCs
Mature Tooth-Supportive Tissues-Stem Cell Lineages
Periodontal Ligament Stem Cells: PDLSCs
Gingiva-Derived Mesenchymal Stem Cells: GMSCs
Donor
Election of Research Method
Bioethics, Legislation and Regulation of Human Stem Cell Research
Dental-Related Tissue Extraction and Transport
Dental-Related Tissues Extraction
Dental-Related Tissues Transport
Isolation of the Dental-Related Tissues
Tooth-Organ Tissues Extraction
Immature Tooth-Organ Tissues Extraction
Dental Papilla and Apical Papilla Extraction
Mature Tooth-Organ Tissues Extraction
Dental Pulp Extraction
Tooth-Supportive Soft Tissues Extraction
Immature Tooth-Supportive Tissues Extraction
Dental Follicle Extraction
Mature Tooth-Supportive Tissues Extraction
Periodontal Ligament and Gingiva Extraction
Dental-Related Stem Cells Isolation
Enzymatic Digestion/Dissociation: ED
Spontaneous Outgrowth/Explant Culture: OG
Dental-Related Stem Cells Amplification and Differentiation Culture Systems
Amplification and Differentiation Culture Systems
Adherent Culture Systems
Non-adherent/Suspension/Sphere-Forming Culture Systems
Co-culture Systems
Amplification Media
Serum-Free Media
Allogeneic Serum-Containing Amplification Media
Xenogeneic Serum-Containing Amplification Media
Dental-Related Stem Cells Differentiation and Evaluation
Osteo-Differentiation Protocol and Evaluation
Chondro-Differentiation Protocol and Evaluation
Adipo-Differentiation Protocol and Evaluation
Neuro-Differentiation Protocol and Evaluation
Myogenic-Differentiation Protocol and Evaluation
Hepatogenic-Differentiation Protocol and Evaluation
Dental-Related Stem Cells Cryopreservation
References
Isolation and Cryopreservation of Stem Cells from Dental Tissues
Introduction
Principles of Cryopreservation
Dental Stem Cell Banking
Banking Intact Whole Teeth
International Licensed Dental Stem Cell Banks
Cryopreservation Procedure
Tooth Collection
Cell Isolation and Expansion
Introduction of a Cryopreservation Solution
Freezing Protocol
Controlled-Rate Freezing
Uncontrolled-Rate Freezing
Rapid Freezing (Vitrification)
Magnetic Freezing
Thawing Conditions
Post-Thaw Assessment
Conclusions
References
Epigenetics of Dental Stem Cells
Introduction
Review
Dental Stem Cells
Epigenetic Modifications and Dental Stem Cells
Histone Acetylation Related Stem Cell Effects
HDACi-Induced Stem Cell Effects
Induced Pluripotent Stem Cells (iPSCs)
Conclusions
References
Dental Stem Cells and Growth Factors
Stem Cells, Scaffolds and Growth Factors
Definition and Mechanism of Action of Growth Factors (GFs)
Dental Stem Cells and Growth Factors
Human Recombinant and Autogenous Growth Factors
Effect of Growth Factors on Dental Stem Cells
Platelet-Derived Growth Factor (PDGF)
Transforming Growth Factor-β (TGFβ)
Bone Morphogenetic Protein (BMP)
Fibroblast Growth Factor (FGF)
Insulin-Like Growth Factor (IGF)
Vascular Endothelial Growth Factor (VEGF)
References
Nano Surface and Stem Cells for Implants
Introduction
Biomaterials
Desired Properties of Biomaterials
Mechanical Properties
Biocompatibility
High Corrosion and Wear Resistance
Osseointegration
Impact of Nano Topography in Implants
Methods of Imparting Nano-Features
Influence of Implant Surface on Osseointegration
Nanotechnology and Cellular Activity
Protein Adsorption (Surface Wettability) in Nano-Surface
Cell Adhesion, Spreading and Motility in Nanosurface
Proliferation in Nano-Surface
Selectivity of Adhesion in Nano-Surface
Differentiation
Nano-Surface and Bacterial Proliferation
Nano-Surface and Surface Reactivity
Biomechanics of Stem Cells and Nano Surface
Nano Topography Supports Stem Cell Differentiation
Interactions Between Implant Surface and Mesenchymal Stem Cells
Interaction of Stem Cells to Nano Surface Dental Implants
Cellular Attachment and Adhesion
Cellular Migration and Proliferation
Cellular Differentiation, Gene Expression and Protein Synthesis
Conclusion
References
Dental Pulp Stem Cells and Hydrogel in Pulp Regeneration
Dental Pulp Stem Cells
Scaffolds
Scaffolds in Dental Pulp Tissue Engineering
Hydrogels
DPSCs and Hydrogels in Dental Pulp Regeneration
Summary
References
Dental Stem Cells for Pulp Regeneration
Introduction
Dental Pulp Tissue Engineering
Dental Stem Cells
Dental Stem Cells-Based Pulp Tissue Engineering
Future Challenges
References
Stem Cells for Periodontal Regeneration
Background
The Importance of Maintaining an Adequate Oral Health Status
Periodontal Diseases
Challenges in Periodontal Tissue Regeneration
Periodontal Regeneration: State of the Art
Biomaterials in Periodontal Regeneration
Tissue Engineering in Periodontal Tissue Regeneration
Protein Therapy: Growth Factor Delivery
Platelet-Rich Plasma
Platelet Derived Growth Factor (PDGF)
Bone Morphogenetic Proteins (BMPs)
Gene Therapy
Cell Therapy
Stem Cells
Applications in Periodontal Regeneration
Future Directions
References
Dental Stem Cells for Tooth Regeneration
Tooth Formation
Molecular Control of Odontogenesis
Signals Specifying Dental Cell Fates
Dental Pathologies
Traditional Dental Treatments and Main Limitations
Alternative Dental Treatments
Stem Cells in Tooth Homeostasis and Regeneration
Stem Cell Populations Within the Dental Pulp
Dental Epithelial Stem Cells
Induced Pluripotent Stem Cells
Experimental Approaches for Tooth Regeneration
Regeneration of Dentin-Pulp Complex
Regeneration of a Whole New Tooth
Conclusion
References
Dental Stem Cells for Bone Regeneration
Introduction
Stem Cells of Dental Tissue Origin
Dental Pulp Stem Cells (DPSCs)
Stem Cells from Human Exfoliated Deciduous Teeth (SHEDs)
Stem Cells from the Apical Papilla (SCAP)
Dental Follicle Stem Cells (DFSCs)
Stem Cells from the Periodontal Ligament (PDLSCs)
Gingival Mesenchymal Stem Cells (GMSCs)
Alveoral-Bone Derived Mesenchymal Stem Cells (ABMSCs)
Tooth Germ Progenitor Cells (TGPCs)
Dental Stem Cells in Craniofacial Bone Healing
Calvarial Bone Defects
Experimental Models of Calvarial Bone Defects
Dental Stem Cells and Bone Repair in the Calvarial Experimental Model
Mandibular and Alveolar Bone Defects
Experimental Models of Mandibular and Alveolar Bone Defects
Dental Stem Cells in the Repair of Mandibular and Alveolar Defects
Dental Stem Cells in Long Bone Healing
Conclusion and Future Perspectives
References
Neuronal Properties of Dental Stem Cells
References
MSCs and Biomaterials
Introduction
Tissue Engineering
Graphene
Digital Dentistry
References
DSC for Ocular Regeneration
Introduction
Dental Stem Cells to Treat Corneal Blindness
Corneal Epithelium
Corneal Stroma
Corneal Endothelium
Dental Stem Cells to Treat Retinal Degeneration
Dental Stem Cells for Retinal Cell Regeneration
Dental Stem Cells to Facilitate Neuroprotection
Conclusions and Future Prospects
References
DSC-Differentiated Hepatocytes for Treatment of Liver Diseases
Introduction
Cellular Therapies for Liver Disease
Effect of Soluble Signals on Hepatic Differentiation of Stem Cells
Various Tissue Sources of DSCs
DSCs-Derived Induced Pluripotent Stem Cells (iPSCs) as Potential Cell Source

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