Culture of Human Stem Cells 1st Edition by Ian Freshney, Glyn Stacey, Jonathan Auerbach ISBN 9780470167519 0470167513
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Authors:R. Ian Freshney; Glyn N. Stacey; Jonathan M. Auerbach , Series:Biochemistry [8] , Tags:Science; Life Sciences; Biochemistry; Biology; Microbiology; 9780470167519.pdf , Author sort:Freshney, R. Ian & Stacey, Glyn N. & Auerbach, Jonathan M. , Ids:Google; 9780470167519 , Languages:Languages:eng , Published:Published:Jul 2007 , Publisher:John Wiley & Sons , Comments:Comments:This book collects the most effective and cutting-edge methods and protocols for deriving and culturing human embryonic and adult stem cells—in one handy resource. This groundbreaking book follows the tradition of previous books in the Culture of Specialized Cells Series—each methods and protocols chapter is laid out exactly like the next, with stepwise protocols, preceded by specific requirements for that protocol, and a concise discussion of methods illustrated by data. The editors describe a limited number of representative techniques across a wide spectrum of stem cells from embryonic, newborn, and adult tissue, yielding an all-encompassing and versatile guide to the field of stem cell biology and culture. The book includes a comprehensive list of suppliers for all equipment used in the protocols presented, with websites available in an appendix. Additionally, there is a chapter on quality control, and other chapters covering legal and ethical issues, cryopreservation, and feeder layer culture. This text is a one-stop resource for all researchers, clinical scientists, teachers, and students involved in this crucial area of study.
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ISBN 10: 0470167513
ISBN 13: 9780470167519
Author: Ian Freshney, Glyn Stacey, Jonathan Auerbach
Culture of Human Stem Cells 1st Edition Table of contents:
Chapter 1: Introduction to Stem Cell Biology
- Overview of Stem Cells
- Types of Stem Cells: Embryonic, Adult, and Induced Pluripotent Stem Cells
- Key Properties of Stem Cells: Self-Renewal, Pluripotency, and Differentiation
- Ethical and Regulatory Issues in Stem Cell Research
Chapter 2: Stem Cell Isolation and Characterization
- Isolation Techniques for Human Stem Cells
- Surface Markers and Cell Sorting Technologies
- Methods of Stem Cell Characterization (e.g., Flow Cytometry, RT-PCR)
- Quality Control and Authentication of Stem Cell Lines
Chapter 3: Stem Cell Culture Media and Supplements
- Basic Culture Media for Stem Cells
- Chemical Supplements for Stem Cell Maintenance
- Serum-Free and Defined Media
- Growth Factors and Cytokines in Stem Cell Culture
Chapter 4: Culture Conditions for Human Stem Cells
- Maintaining Pluripotency: Culture of Embryonic and Induced Pluripotent Stem Cells (iPSCs)
- Strategies for Differentiation of Stem Cells
- Hypoxic Conditions and Their Impact on Stem Cell Culture
- 3D Culture Systems for Stem Cells
Chapter 5: Bioreactors and Scalable Culture Systems
- The Role of Bioreactors in Stem Cell Culture
- Large-Scale Expansion of Stem Cells
- Automation and High-Throughput Stem Cell Culture Systems
- Applications in Tissue Engineering and Regenerative Medicine
Chapter 6: Genetic Manipulation of Human Stem Cells
- Techniques for Transfection and Transduction of Stem Cells
- Gene Editing Tools: CRISPR/Cas9 and TALENs
- Methods for Introducing Reporter Genes
- Ethical Considerations in Genetic Modification of Stem Cells
Chapter 7: Differentiation Protocols for Human Stem Cells
- Directed Differentiation of Stem Cells to Specific Lineages (e.g., Neural, Cardiac, Hepatic)
- Protocols for Differentiating iPSCs into Functional Cell Types
- Advances in Organoid Culture Systems
- Challenges in Differentiation and Maturation of Stem Cell-Derived Tissues
Chapter 8: Stem Cell-Based Disease Models and Drug Screening
- Using Human Stem Cells to Model Diseases In Vitro
- High-Throughput Drug Screening with Stem Cells
- Organ-on-a-Chip Models and Their Applications
- Stem Cells for Toxicity Testing and Personalized Medicine
Chapter 9: Stem Cells in Regenerative Medicine
- Stem Cell Therapies for Tissue Repair and Regeneration
- Clinical Applications: Bone Marrow, Cartilage, and Heart Repair
- Stem Cells in the Treatment of Neurodegenerative Diseases
- Current Challenges and Future Prospects in Regenerative Medicine
Chapter 10: Stem Cells in Immunotherapy and Cancer
- The Role of Stem Cells in Cancer Research
- Immunotherapy Approaches Involving Stem Cells
- Stem Cells in the Tumor Microenvironment
- Cancer Stem Cells and Targeted Therapies
Chapter 11: Stem Cells in Personalized Medicine
- Personalized Approaches to Stem Cell-Based Therapies
- Patient-Specific iPSCs for Disease Modeling and Drug Testing
- Ethical Issues in Personalized Stem Cell Therapy
- The Future of Personalized Medicine with Stem Cells
Chapter 12: Clinical Trials and Regulatory Considerations
- Overview of Clinical Trials Involving Stem Cells
- Regulatory Framework for Stem Cell-Based Therapies
- Manufacturing and Quality Control of Stem Cell Products
- Safety and Efficacy of Stem Cell Clinical Trials
Chapter 13: Commercialization and Biotechnology Applications
- Stem Cells in Biotechnology and Industry
- Commercial Products Derived from Stem Cells
- Business Models in Stem Cell Research and Therapy
- Patents, Intellectual Property, and Market Considerations
Chapter 14: Ethical, Legal, and Social Implications of Stem Cell Research
- Ethical Issues in Human Stem Cell Research
- Legal Framework for Stem Cell-Based Research and Therapy
- Social and Cultural Impacts of Stem Cell Therapies
- Public Perception and Education on Stem Cell Science
Chapter 15: Future Directions in Stem Cell Research and Applications
- Emerging Technologies in Stem Cell Culture
- Advances in Organoid and 3D Tissue Engineering
- The Role of Artificial Intelligence and Big Data in Stem Cell Research
- The Path Forward: Challenges and Opportunities in Stem Cell Science
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