Basic methods in antibody production and characterization 1st Edition by Gary Howard, Delia Bethell ISBN 0849394457 9780849394454
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Authors:Gary C. Howard , Series:Pathology [10] , Tags:1. Immunoglobulins—Laboratory manuals. I. Howard; Gary C. II. Bethell; Delia R. , Author sort:Howard, Gary C. , Languages:Languages:eng , Published:Published:May 2007 , Publisher:CRC Press , Comments:Comments:Antibodies have become indispensable tools for biomedical research. Their extraordinary specificity of binding is matched by their ease of use and adaptability to many experimental and now even some therapeutic applications. Basic Methods in Antibody Production and Characterization is a cookbook of methods for the production and use of antibodies. We have attempted to cover all aspects of poloyclonal and monoclonal antibodies. Many of the procedures are well established. Others (e.g., phage display) are quite new. Although we have sought to present this material in a simple step-by-step manner, no methods book can cover every experimental protocol. Each contributor has provided introductory material to give a basic understanding of the methods and references to other applications of his or her chapter’s particular technology. The methods presented are more or less in chronological order. The most important element in making antibodies is the ultimate purpose of the experiments. Time, experience, expense, and the scientific questions to be answered will direct the choice of methods for antibody production.
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ISBN 10: 0849394457
ISBN 13: 9780849394454
Author: Gary Howard, Delia Bethell
Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies.
Antibodies described include polyclonal and monoclonal and those made by the phage display. Basic Methods in Antibody Production and Characterization focuses on specific aspects of antibodies and takes readers on a step-by-step process from antigen preparation to immunizations, adjuvants, screening, purification, storage, and applications.
Introductory material accompanies each chapter, giving readers a better understanding of the methods and applications. Ample references arm researchers with many applications pertaining to the particular technology.
Basic methods in antibody production and characterization 1st Edition Table of contents:
1. Introduction to Antibody Production
- Overview of Antibodies and Their Importance
- Types of Antibodies: Polyclonal and Monoclonal
- Applications of Antibodies in Research, Diagnostics, and Therapy
- Principles Behind Antibody Generation
2. Antibody Generation: Polyclonal Antibodies
- Immunization Strategies and Techniques
- Selection of Immunogens and Adjuvants
- Blood Collection and Serum Preparation
- Screening and Characterization of Polyclonal Antibodies
3. Monoclonal Antibody Production
- Principles of Hybridoma Technology
- Fusion of B Cells and Myeloma Cells
- Cloning and Selection of Hybridomas
- Screening, Characterization, and Expansion of Hybridoma Clones
4. Recombinant Antibodies
- Introduction to Recombinant Antibody Technologies
- Construction of Antibody Libraries
- Phage Display Technology and Antibody Selection
- Expression and Purification of Recombinant Antibodies
5. Antibody Purification Methods
- Methods for Purifying Antibodies from Serum and Supernatant
- Affinity Chromatography for Antibody Purification
- Protein A/G Affinity Columns and Their Use
- Dialysis, Ultrafiltration, and Ion Exchange Chromatography
6. Antibody Labeling and Conjugation
- Techniques for Labeling Antibodies with Enzymes, Fluorochromes, and Radioisotopes
- Conjugation of Antibodies to Beads, Nanoparticles, and Other Targets
- Strategies for Conjugating Antibodies to Therapeutic Agents
7. Characterization of Antibodies
- Determining Antibody Specificity and Affinity
- ELISA, Western Blotting, and Immunoprecipitation for Antibody Characterization
- Measuring Antibody Titer and Functional Activity
- Cross-reactivity and Antibody Epitope Mapping
8. Antibody Validation and Quality Control
- Ensuring Antibody Specificity and Sensitivity
- Assessing the Performance of Antibodies in Different Applications
- Criteria for Selecting and Validating Commercial Antibodies
- Troubleshooting Common Problems with Antibody Use
9. In Vivo Applications of Antibodies
- Therapeutic Antibodies and Their Role in Disease Treatment
- Antibodies in Cancer Immunotherapy
- Antibody-Based Diagnostics and Imaging
- Immunoglobulin Engineering for Specific In Vivo Targets
10. Antibody Applications in Research
- Antibodies in Immunohistochemistry and Immunofluorescence
- Flow Cytometry and Antibody Labeling
- Antibodies in Cell Signaling and Protein Interaction Studies
- Application in Animal Models and Disease Research
11. Ethical and Regulatory Considerations in Antibody Production
- Ethical Issues in the Production of Antibodies, including Animal Use
- Regulatory Requirements for Antibody-based Products
- Good Manufacturing Practices (GMP) for Antibody Production
- Guidelines for Safe Handling and Storage of Antibodies
12. Future Directions in Antibody Technology
- Advances in Antibody Engineering and Humanization
- Antibody Therapeutics: Challenges and Opportunities
- Nanobodies and Single-Domain Antibodies
- The Role of Artificial Intelligence in Antibody Design
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