Apoptosis Techniques and Protocols 2nd Edition by Andréa LeBlanc ISBN 9781592591886 159259188X
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Authors:andrea, C Leblanc , Series:Pathology [5] , Tags:Pathology , Author sort:andrea, C Leblanc , Languages:Languages:eng , Published:Published:Oct 2007 , Publisher:Humana Press , Comments:Comments:When the President of Humana Press first suggested that a series on methods in the neurosciences might be useful, one of us (AAB) was quite skeptical; only after discussions with GBB and some searching both of memory and library shelves did it seem that perhaps the publisher was right. Although some excellent methods books had recently appeared, notably in neuroanatomy, it was a fact that there was a dearth in this particular field, a fact attested to by the alacrity and enthusiasm with which most of the contributors to this series accepted our invitations and suggested additional topics and areas. After a somewhat hesitant start, essentially in the neurochemistry section, the series has grown and will encompass neurochemistry, neuropsychiatry, neurology, neuropathology, neurogenetics, neuroethology, molecular neurobiology, animal models of nervous disease, and no doubt many more “neuros.†Although we have tried to include adequate methodological detail and in many cases detailed protocols, we have also tried to include wherever possible a short introductory review of the methods and/or related substances, comparisons with other methods, and the relationship of the substances being analyzed to neurological and psychiatric disorders. Recognizing our own limitations, we have invited a guest editor to join with us on most volumes in order to ensure complete coverage of the field. These editors will add their specialized knowledge and competencies. We anticipate that this series will fill a gap; we can only hope that it will be filled appropriately and with the right amount of expertise with respect to each method, substance or group of substances, and area treated.h
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ISBN 10: 159259188X
ISBN 13: 9781592591886
Author: Andréa LeBlanc
The study of the apoptotic process has grown exponentially since the publication of the first edition of Apoptosis Techniques and Protocols for the Neuromethods series in 1997. In this greatly updated second edition, seasoned experts describe in step-by-step detail their best state-of-the-art techniques for studying neuronal cell death. These readily reproducible methods solve a wide variety of research problems, including the detection of the key proteins involved in neuronal apoptosis (Bax protein, cytochrome-c, and caspases), the direct assessment of the role of pro-apoptotic proteins in neurons by viral infections and microinjections, and the detection of proapoptotic proteins in situ. There are also hands-on methods for the study of apoptosis mechanisms in neuronal compartments, for studying synaptosis, and for establishing gene expression profiles in neurodegenerative brain tissues by using DNA microarrays. Written by investigators who have used the techniques extensively, each protocol includes tips on avoiding pitfalls, notes on the method’s advantages and disadvantages, and a critical survey of the literature.
Cutting-edge and highly practical, Apoptosis Techniques and Protocols, Second Edition, offers both novice and seasoned investigators a rich panoply of the productive tools they need to unravel the molecular mechanisms of neuronal cell death.
Apoptosis Techniques and Protocols 2nd Edition Table of contents:
1. Introduction to Apoptosis
- Overview of Apoptosis
- Mechanisms and Pathways of Apoptosis
- Importance of Apoptosis in Disease and Therapy
2. Basic Assays to Detect Apoptosis
- Morphological Criteria for Apoptosis Detection
- Flow Cytometry-based Detection of Apoptosis
- DNA Fragmentation Assays
- Caspase Activity Assays
- Annexin V/PI Staining Method for Apoptosis Detection
3. Measuring Mitochondrial Changes in Apoptosis
- Mitochondrial Membrane Potential Assays
- Cytochrome c Release Assays
- Mitochondrial ROS Production in Apoptosis
- Evaluation of Mitochondrial Permeability Transition
4. Caspase Activation and Inhibition
- Detection of Caspase Activation Using Fluorogenic Substrates
- Inhibition of Caspases in Apoptosis Studies
- Monitoring Caspase Activity by Western Blotting and ELISA
- Caspase Protease Assays
5. DNA Fragmentation and Nuclear Changes
- TUNEL Assay for Detecting DNA Fragmentation
- Hoechst Staining for Nuclear Condensation
- Chromatin Condensation and Nuclear Fragmentation Analysis
6. Protein Markers of Apoptosis
- Detection of Cleaved PARP (Poly ADP-Ribose Polymerase)
- Measurement of Bax, Bcl-2, and Other Apoptosis-Related Proteins
- Western Blotting for Apoptotic Protein Expression
- Immunofluorescence and Immunohistochemistry Techniques
7. In Vivo and Ex Vivo Models for Apoptosis Studies
- Animal Models of Apoptosis and Cell Death
- Ex Vivo Cultured Tissue Models for Studying Apoptosis
- Measuring Apoptosis in Tissues Using Histological Staining
8. Apoptosis in Disease Models
- Apoptosis in Cancer Research
- Neurodegenerative Diseases and Apoptosis
- Apoptosis in Cardiac and Renal Diseases
- Apoptosis in Immune Disorders and Inflammatory Diseases
9. Methods for Inhibiting Apoptosis
- Pharmacological Inhibitors of Apoptosis
- Genetic Manipulation of Apoptotic Pathways
- RNA Interference (RNAi) to Modulate Apoptosis
- Overexpression and Knockdown Techniques
10. Apoptosis and Cancer Therapeutics
- Targeting Apoptotic Pathways for Cancer Treatment
- Chemotherapeutic Agents that Induce Apoptosis
- Small Molecule Inhibitors of Apoptotic Proteins
- Immunotherapy and Apoptosis
11. High Throughput Screening of Apoptosis
- Automated Methods for Apoptosis Detection
- Screening for Apoptotic Compounds Using Cell-Based Assays
- Fluorescent and Luminescent Assay Systems
- Quantitative Methods for Apoptosis Detection
12. Data Analysis and Interpretation in Apoptosis Studies
- Analyzing Flow Cytometry Data
- Statistical Methods for Apoptosis Research
- Troubleshooting Common Pitfalls in Apoptosis Assays
13. Future Directions in Apoptosis Research
- Emerging Technologies for Apoptosis Detection
- Role of Apoptosis in Regenerative Medicine
- Targeting Apoptotic Pathways in Drug Development
- New Insights in Apoptosis Mechanisms
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