Analytical Biochemistry 3rd Edition by David Holme, Hazel Peck ISBN 058229438X 9780582294387

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Product details:

ISBN 10:       058229438X
ISBN 13: 978-0582294387
Author:        David J. Holme, Hazel Peck

 Now an established handbook of principles and techniques, this text develops an understanding of the relevance of four fundamental properties of the analyte: shape, polarity, charge and size, to the three key types of analysis: separation, identification and quantification. The third edition of Analytical Biochemistry has now been fully updated in content and format, making it even more accessible to students learning how to select analytical techniques and recognise their scope and limitations.

Analytical Biochemistry 3rd Table of contents:

1. General Principles of Analytical Biochemistry

• Selection of a Valid Method of Analysis
• Quality of Data
• Production of Results

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2. Spectroscopy

• Interaction of Radiation with Matter
• Molecular Absorptiometry
  • Absorptiometer Design
• Molecular Fluorescence Techniques
• Atomic Spectroscopy Techniques
• Magnetic Resonance Spectroscopy

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3. Separation Methods

• Principles of Separation Techniques
• Methods Based on Polarity
• Methods Based on Ionic Nature
• Methods Based on Size
• Methods Based on Shape

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4. Electroanalytical Methods

• Potentiometry
• Conductimetry
• Coulometry
• Voltammetry
• Biosensors

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5. Radioisotopes

• Nature of Radioactivity
• Detection and Measurement of Radioactivity
• Biochemical Use of Isotopes

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6. Automated Methods of Analysis

• Discrete Analyzers
• Flow Analysis
• Robotics

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7. Immunological Methods

• General Processes of the Immune Response
• Antigen-Antibody Reactions
• Analytical Techniques:
  • Precipitation Reactions
  • Immunoassay

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8. Enzymes

• Nature of Enzymes
• Enzyme Assay Methods
• Monitoring Techniques
• Treatment of Samples for Enzyme Assay
• Substrate Assay Methods
• Automated Analysis
• Immobilized Enzymes

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9. Carbohydrates

• General Structure and Function
• Chemical Methods of Carbohydrate Analysis
• Enzymic Methods of Carbohydrate Analysis
• Separation and Identification of Carbohydrate Mixtures

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10. Amino Acids

• General Structure and Properties
• General Reactions
• N-terminal Analysis
• Reactions of Specific Amino Acids
• Separation of Amino Acid Mixtures
• Amino Acid Analyzer

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11. Proteins

• Protein Structure
• General Methods of Quantitation
• Separation of Proteins

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12. Lipid-Protein Structures

• Sample Preparation and Handling
• Quantitative Methods
• Separation of Lipid Mixtures

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13. Nucleic Acids

• Nucleic Acid Composition and Structure
• Isolation and Purification of Nucleic Acids
• Methods of Nucleic Acid Analysis
• Vectors
• DNA Sequencing

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