Alzheimer’s Disease Methods and Protocols 1st Edition by Nigel Hooper ISBN 1617371610 9781617371615

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ISBN 10: 1617371610

ISBN 13: 978-1617371615

Author:  Nigel M. Hooper 

Alzheimer’s Disease: Methods and Protocols 1st Edition: 

Alzheimer’s Disease: Methods and Protocols 1st Edition Table of contents:

1 Introduction to Alzheimer’s Disease
2 The Genetics of Alzheimer’s Disease
3 Advances in Methodology and Current Prospects for Primary Drug Therapies for Alzheimer’s Disease
4 Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (SAPP)
5 Quantifying Aβ1-40 and Aβ1-42 Using Sandwich-ELISA
6 Electrophoretic Separation and Immunoblotting of Aβ1-40 and Aβ1-42
7 Aβ-Induced Proinflammatory Cytokine Release from Differentiated Human THP-1 Monocytes
8 Effects of the β-Amyloid Peptide on Membrane Ion Permeability
9 Analysis of β-Amyloid Peptide Degradation In Vitro
10 Posttranslational Modifications of the Amyloid Precursor Protein: Ectodomain Phosphorylation and Sulfation
11 Posttranslational Modifications of the Amyloid Precursor Protein: Glycosylation
12 Using an Amyloid Precursor Protein (APP) Reporter to Characterize α-Secretase
13 Inhibition of α-Secretase by Zinc Metalloproteinase Inhibitors
14 Development of Neoepitope Antibodies Against the β-Secretase Cleavage Site in the Amyloid Precursor Protein
15 β-Secretase: Tissue Culture Studies of Sequence Specificity, Inhibitors, and Candidate Enzymes
16 Using Secretase Inhibitors to Distinguish the Generation of the Aβ Peptides Terminating at Val-40 and Ala-42

17 Designing Animal Models of Alzheimer’s Disease with Amyloid Precursor Protein (APP) Transgenes
18 Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins
19 Determining the Transmembrane Topology of the Presenilins
20 Normal Proteolytic Processing of the Presenilins
21 Apoptotic Proteolytic Cleavage of the Presenilins by Caspases
22 The Phosphorylation of Presenilin Proteins
23 Interaction of the Presenilins with the Amyloid Precursor Protein (APP)
24 Distribution of Presenilins and Amyloid Precursor Protein (APP) in Detergent-Insoluble Membrane Domains
25 Characterization and Use of Monoclonal Antibodies to Tau and Paired Helical Filament Tau
26 Tau Phosphorylation Both In Vitro and in Cells
27 Transglutaminase-Catalyzed Formation of Alzheimer-Like Insoluble Complexes from Recombinant Tau

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