A Guide to Methods in the Biomedical Sciences 1st Edition by Ronald Corley ISBN 9780387228457 0387228454

A Guide to Methods in the Biomedical Sciences gives a basic description of common methods used in research. This is not intended to be a methods book. Rather, it is intended to be a book that outlines the purpose of the methods described, their limitations and provide alternative approaches as appropriate. Thousands of methods have been developed in the various biomedical disciplines and those covered in this book represent the basic, essential and most widely used methods in several different disciplines. The historical background (including some interesting anecdotes) leading to the development of ground-breaking techniques is described, especially of those that significantly advanced the field of biomedical research. Advances that earned their inventors prestigious Nobel Prizes are emphasized. The book is divided into six sections, highlighting selected methods in protein chemistry, nucleic acids, recombinant DNA technology (including forensic-based methods), antibody-based techniques, microscopy and imaging, and the use of animals in biomedical sciences.

 

A Guide to Methods in the Biomedical Sciences 1st Edition Table of contents:

Chapter 1: Introduction to Biomedical Research

• Overview of Biomedical Sciences
• The Scientific Method and Experimental Design
• Ethical Considerations in Biomedical Research
• Safety Protocols and Laboratory Practices
• Preparing for a Successful Research Project

Chapter 2: Molecular Biology Techniques

• DNA Extraction and Purification Methods
• Polymerase Chain Reaction (PCR) and Variants
• Gel Electrophoresis for DNA, RNA, and Protein Analysis
• Cloning and Transformation Techniques
• Gene Editing and CRISPR-Cas9 Technology

Chapter 3: Protein Biochemistry

• Protein Extraction and Purification
• Techniques for Protein Quantification
• SDS-PAGE and Western Blotting for Protein Analysis
• Enzyme-Linked Immunosorbent Assay (ELISA)
• Mass Spectrometry in Protein Characterization
• Protein-Protein Interaction Studies

Chapter 4: Cell Culture Techniques

• Basics of Mammalian Cell Culture
• Cell Line Maintenance and Subculturing
• Primary Cells and Stem Cell Cultures
• Transfection Methods: DNA and RNA Delivery
• Assessing Cell Viability and Proliferation
• Cell Differentiation and Transformation Assays

Chapter 5: Microscopy and Imaging Techniques

• Light Microscopy: Principles and Techniques
• Fluorescence Microscopy and Applications
• Confocal and Electron Microscopy
• Live-Cell Imaging and Fluorescence Recovery After Photobleaching (FRAP)
• Imaging for Cell and Tissue Analysis
• Quantification and Image Analysis Software

Chapter 6: Flow Cytometry and Cell Sorting

• Basic Principles of Flow Cytometry
• Fluorescent Antibodies and Cell Markers
• Analyzing Cell Populations and Subsets
• Cell Sorting and Isolation Methods
• Applications of Flow Cytometry in Immunology and Hematology

Chapter 7: Animal Models in Biomedical Research

• Choosing the Right Animal Model for Your Study
• Genetically Engineered Mouse Models
• In Vivo Imaging Techniques
• Behavioral Assessments in Animal Research
• Ethical Issues and Regulations in Animal Research
• Animal Models in Disease Research

Chapter 8: Immunology and Immunohistochemistry

• Techniques for Antibody Production and Purification
• Immunohistochemistry (IHC) and Immunofluorescence
• ELISA and Luminex Assays
• Flow Cytometric Immunophenotyping
• Cytokine Detection and Immune Cell Profiling

Chapter 9: Clinical and Diagnostic Techniques

• Clinical Biochemistry: Blood, Urine, and Tissue Analysis
• Diagnostic Imaging Methods: X-ray, MRI, CT
• Point-of-Care Diagnostics and Devices
• Diagnostic Microbiology: Culturing and Identifying Pathogens
• PCR-Based Diagnostic Tests
• Biomarker Discovery and Validation

Chapter 10: Genomics and Bioinformatics

• Next-Generation Sequencing (NGS) Techniques
• RNA Sequencing and Transcriptomics
• Genome-Wide Association Studies (GWAS)
• Bioinformatics Tools for Data Analysis
• Interpreting Genomic Data for Disease Research
• Applications of Genomics in Personalized Medicine

Chapter 11: Drug Discovery and Pharmacology

• High-Throughput Screening Methods
• Drug-Receptor Interaction Studies
• Pharmacokinetics and Pharmacodynamics
• Animal Models for Drug Testing
• In Vitro Toxicology Assays
• Drug Development and Clinical Trials

Chapter 12: Statistical Methods in Biomedical Research

• Principles of Experimental Design
• Statistical Analysis for Biomedical Research
• Data Collection and Interpretation
• Software for Statistical Analysis: SPSS, R, and More
• Biostatistics in Clinical Trials
• Reporting and Presenting Statistical Data

Chapter 13: Writing and Presenting Scientific Research

• Writing a Scientific Paper: Structure and Style
• Grant Writing and Funding for Research
• Presenting Research Findings: Posters and Presentations
• Preparing for Peer Review and Publication
• Ethical Considerations in Scientific Publishing

Appendix A: Laboratory Safety Guidelines

• Chemical and Biological Safety Protocols
• Personal Protective Equipment (PPE)
• Emergency Procedures and First Aid
• Waste Disposal and Environmental Health

Appendix B: Key Biomedical Science Resources

• Key Journals and Publications in Biomedical Sciences
• Online Databases and Research Tools
• Organizations and Professional Societies
• Research Funding Agencies

People also search for A Guide to Methods in the Biomedical Sciences 1st Edition: