Physical Biochemistry Principles and Applications 2nd Edition by David Sheehan 0470856033 9780470856031
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Authors:David Sheehan , Series:Biochemistry [81] , Tags:Science; Life Sciences; Biochemistry; Chemistry; Physical & Theoretical; Microbiology , Author sort:Sheehan, David , Ids:9780471986638 , Languages:Languages:eng , Published:Published:Jun 2000 , Publisher:Wiley , Comments:Comments:Physical Biochemistry: Principles and Applications provides a broad overview of the key techniques currently used to characterise the structure and function of complex biomacromolecules such as proteins and DNA. It bridges the gap between general biochemistry textbooks and the more specialist texts covering individual techniques. Topics covered include chromatography, spectroscopy, mass spectrometry , electrophoresis, X-ray diffraction, centrifugation and biocalorimetry. New developments are placed in context by describing the physical principles on which they depend, examining the range of biophysical applications most widely used and, emphasising the overall similarities of experimental approach. Written by a biochemist with extensive teaching experience, the book: * describes the advantages and disadvantages of each technique and compares one technique to another; * introduces experimental approaches in a non-mathematical way, using practical examples; and, * provides a bibliography, including useful web sites, at the end of each chapter. Physical Biochemistry: Principles and Applications will be invaluable to undergraduates and postgraduates studying biochemistry, molecular biology and related disciplines.
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ISBN 10: 0470856033
ISBN 13: 9780470856031
Author: David Sheehan
“As will be seen, there is not much missing here. I thought that the sections were well balanced, with rarely too much or too little on a given topic…This is a text to be welcomed by both teachers and students.” BIOCHEMISTRY & MOLECULAR BIOLOGY EDUCATION (on the first edition) The second edition of this successful textbook explains the basic principles behind the key techniques currently used in the modern biochemical laboratory and describes the pros and cons of each technique and compares one to another. It is non-mathematical, comprehensive and approachable for students who are not physical chemists. A major update of this comprehensive, accessible introduction to physical biochemistry. Includes two new chapters on proteomics and bioinformatics. Introduces experimental approaches with a minimum of mathematics and numerous practical examples. Provides a bibliography at the end of each chapter. Written by an author with many years teaching and research experience, this text is a must-have for students of biochemistry, biophysics, molecular and life sciences and food science.
Physical Biochemistry Principles and Applications 2nd Table of contents:
Chapter 1 Introduction
1.1 Special Chemical Requirements of Biomolecules
1.2 Factors Affecting Analyte Structure and Stability
1.3 Buffering Systems Used in Biochemistry
1.4 Quantitation, Units and Data Handling
1.5 The World Wide Web as a Resource in Physical Biochemistry
1.6 Objectives of this Volume
Chapter 2 Chromatography
2.1 Principles of Chromatography
2.2 Performance Parameters Used in Chromatography
2.3 Chromatography Equipment
2.4 Modes of Chromatography
2.5 Open Column Chromatography
2.6 High Performance Liquid Chromatography (HPLC)
2.7 Fast Protein Liquid Chromatography
2.8 Perfusion Chromatography
2.9 Membrane-Based Chromatography Systems
2.10 Chromatography of a Sample Protein
Chapter 3 Spectroscopic Techniques
3.1 The Nature of Light
3.2 The Electromagnetic Spectrum
3.3 Ultraviolet/Visible Absorption Spectroscopy
3.4 Fluorescence Spectroscopy
3.5 Spectroscopic Techniques Using Plane-Polarized Light
3.6 Infrared Spectroscopy
3.7 Nuclear Magnetic Resonance (NMR) Spectroscopy
3.8 Electron Spin Resonance (ESR) Spectroscopy
3.9 Lasers
3.10 Surface Plasmon Resonance
Chapter 4 Mass Spectrometry
4.1 Principles of Mass Spectrometry
4.2 Mass Spectrometry of Proteins/Peptides
4.3 Interfacing MS With other Methods
4.4 Uses of Mass Spectrometry in Biochemistry
Chapter 5 Electrophoresis
5.1 Principles of Electrophoresis
5.2 Nondenaturing Electrophoresis
5.3 Denaturing Electrophoresis
5.4 Electrophoresis in DNA Sequencing
5.5 Isoelectric Focusing (IEF)
5.6 Immunoelectrophoresis
5.7 Agarose Gel Electrophoresis of Nucleic Acids
5.8 Pulsed Field Gel Electrophoresis
5.9 Capillary Electrophoresis
5.10 Electroblotting Procedures
5.11 Electroporation
Chapter 6 Three-Dimensional Structure Determination of Macromolecules
6.1 The Protein-Folding Problem
6.2 Structure Determination by NMR
6.3 Crystallization of Biomacromolecules
6.4 X-Ray Diffraction by Crystals
6.5 Calculation of Electron Density Maps
6.6 Other Diffraction Methods
6.7 Comparison of X-Ray Crystallography with Multi-Dimensional NMR
6.8 Structural Databases
Chapter 7 Hydrodynamic Methods
7.1 Viscosity
7.2 Sedimentation
7.3 Methods for Varying Buffer Conditions
7.4 Flow Cytometry
Chapter 8 Biocalorimetry
8.1 The Main Thermodynamic Parameters
8.2 Isothermal Titration Calorimetry
8.3 Differential Scanning Calorimetry
8.4 Determination of Thermodynamic Parameters by Non-Calorimetric Means
Chapter 9 Bioinformatics
9.1 Overview of Bioinformatics
9.2 Sequence Databases
9.3 Tools for Analysis of Primary Structures
9.4 Tertiary Structure Databases
9.5 Programs for Analysis and Visualization of Tertiary Structure Databases
9.6 Homology Modelling
Chapter 10 Proteomics
10.1 Electrophoresis in Proteomics
10.2 Mass Spectrometry in Proteomics
10.3 Chip Technologies in Proteomics
10.4 Post-Translational Modification Proteomics
Appendix 1 SI Units
Appendix 2 The Fourier Transform
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