Experimental Biochemistry theory and exercise in fundamental methods 3rd Edition by Robert Switzer, Liam Garrity ISBN 9780716733003 0716733005

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ISBN 10: 0716733005
ISBN 13: 9780716733003
Author: Robert Switzer, Liam Garrity
 a comprehensive introduction to the essential techniques and principles used in biochemistry research. The book offers a blend of theoretical insights and practical exercises designed to enhance students’ understanding of core laboratory methods, such as protein purification, enzyme kinetics, and molecular analysis. The third edition includes updated protocols, new experiments, and modern developments in biochemistry, making it a valuable resource for students and researchers aiming to master key laboratory techniques in biochemistry.

Experimental Biochemistry theory and exercise in fundamental methods 3rd Edition Table of contents:

Section I: Basic Techniques of Experimental Biochemistry

• Introduction
• Experiment 1: Photometry
• Experiment 2: Chromatography
• Experiment 3: Radioisotope Techniques
• Experiment 4: Electrophoresis

Section II: Proteins and Enzymology

• Introduction
• Experiment 5: Acid-Base Properties of Amino Acids
• Experiment 6: Sequence Determination of a Dipeptide
• Experiment 7: Study of the Properties of ß-Galactosidase
• Experiment 8: Purification of Glutamate Oxaloacetate Transaminase from Pig Heart
• Experiment 9: Kinetic and Regulatory Properties of Aspartate Transcarbamylase
• Experiment 10: Affinity Purification of Glutathione-S-Transferase

Section III: Biomolecules and Biological Systems

• Introduction
• Experiment 11: Microanalysis of Carbohydrate Mixtures by Isotopic Enzymatic, and Colorimetric Methods
• Experiment 12: Glucose-1-Phosphate: Enzymatic Formation from Starch and Chemical Characterization
• Experiment 13: Isolation and Characterization of Erythrocyte Membranes
• Experiment 14: Electron Transport
• Experiment 15: Study of the Phosphoryl Group Transfer Cascade Governing Glucose Metabolism; Allosteric and Covalent Regulation of Enzyme Activity
• Experiment 16: Experiments in Clinical Biochemistry and Metabolism

Section IV: Immunochemistry

• Introduction
• Experiment 17: Partial Purification of a Polyclonal Antibody, Determination of Titer, and Quantitation of an Antigen Using the ELISA
• Experiment 18: Western Blot to Identify an Antigen

Section V: Nucleic Acids

• Introduction
• Experiment 19: Isolation of Bacterial DNA
• Experiment 20: Transformation of a Genetic Character with Bacterial DNA
• Experiment 21: Constructing and Characterizing a Recombinant DNA Plasmid
• Experiment 22: In Vitro Transcription from a Plasmid Carrying a T7 RNA Polymerase-Specific Promoter
• Experiment 23: In Vitro Translation: mRNA, tRNA, and Ribosomes
• Experiment 24: Amplification of a DNA Fragment Using Polymerase Chain Reaction

Section VI: Information Science

• Introduction
• Experiment 25: Obtaining and Analyzing Genetic and Protein Sequence Information via the World Wide Web, Lasergene, and RasMol

Appendix

• Supplies and Reagents
• Index

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