Biochemistry in the Lab: A Manual for Undergraduates 1st Edition by Benjamin Lasseter ISBN 0429957378 9780429957376

Biochemistry in the Lab: A Manual for Undergraduates 1st Edition by  Benjamin F. Lasseter – Ebook PDF Instant Download/Delivery. 0429957378, 978-0429957376
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Product details:

ISBN 10:       0429957378
ISBN 13: 978-0429957376
Author:        Benjamin F. Lasseter

Biochemistry in the Lab: A Manual for Undergraduates 1st Table of contents:

Chapter Summaries:

1. Buffers:

   • Methods for creating buffers using acids and bases.
   • Key focus on titrating a free acid with NaOH or a free base with HCl and adding both acid and base to make a buffer.
   • Covers concentrated buffers and questions to test understanding.

2. Assays:

   • Includes specific assays for enzymes such as Lactate Dehydrogenase, Phosphofructokinase, Catalase, and others.
   • Emphasis on enzyme activity reporting and dilutions.
   • Evaluating the source of enzymes for assays.

3. Protein Concentration:

   • Techniques to determine protein concentration, such as absorbance at 280 nm and various assays like the Bradford Assay.
   • Questions on how to calculate protein concentration.

4. ELISA (Enzyme-Linked Immunosorbent Assay):

   • Focuses on the procedure for conducting an ELISA, a method for detecting specific proteins.

5. Salting Out Proteins:

   • Method for protein precipitation by salting out.
   • Includes data analysis for understanding the results.

6. Isoelectric Point and Effective Charge:

   • Discussion on the effective charge of proteins and their isoelectric points, explaining their roles in protein behavior.

7. Column Chromatography:

   • Types and applications of column chromatography in protein separation.
   • Discusses issues in chromatography and backbone interactions.

8. Michaelis-Menten Kinetics:

   • Analysis of enzyme kinetics, including cooperativity and the Michaelis-Menten equation.
   • Experimental procedures for catalase assays.

9. Protein Purification:

   • Covers protein purification through dialysis and other techniques across multiple lab sessions.

10. Polyacrylamide Gels:

    • Detailed guide on using polyacrylamide gel electrophoresis (SDS-PAGE) for protein separation.

11. In Silico Biochemistry:

    • Introduction to computational biochemistry, including the evolution of globins and sequence alignment tools.

12. Growing Crystals of Hemoglobin:

    • Techniques for growing protein crystals using hanging drop and sitting drop methods.
    • Refining crystal growth for structural studies.

13. Enzyme Inhibition:

    • Study of enzyme inhibition mechanisms like competitive, uncompetitive, and mixed inhibition.
    • Includes assays on acid phosphatase activity.

14. Multisubstrate Kinetics:

    • Experimental design for studying product inhibition in enzyme reactions.

15. Fluorescence and Denaturation:

    • Techniques to study protein denaturation using fluorescence, including FRET studies.

16. Fluorescence Studies of Ligand Binding:

    • Focus on experimental designs to study ligand binding through fluorescence.

17. DNA Restriction Digests:

    • An introduction to restriction enzymes and their role in gene expression and DNA manipulation.
    • Instructions for performing restriction digests and constructing restriction maps.

18. Western Blotting:

    • Protocols and materials for performing Western blotting to detect specific proteins.

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