Making and Using Antibodies A Practical Handbook 1st Edition by Gary Howard, Matthew Kaser ISBN 0849335280 9780849335280
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Authors:Gary C. Howard; Matthew R. Kaser , Series:Pathology [9] , Tags:Pathology; Microbiology , Author sort:Howard, Gary C. & Kaser, Matthew R. , Ids:9781138406933 , Languages:Languages:eng , Published:Published:Feb 2013 , Publisher:CRC Press , Comments:Comments:”This second edition provides guidance on all aspects of how to make and use antibodies for research, and commercial and industrial applications. All chapters are revised, updated, or expanded. New material in the book includes western blotting, aptamers, antibodies as therapeutics, quantitative production, and humanization of antibodies. The authors present descriptions of basic methods for making and using antibodies and describe basic laboratory techniques in detail. Each chapter includes introductory material, allowing for a better understanding of each concept. Practical examples are also included”–Provided by publisher.
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Product details:
ISBN 10: 0849335280
ISBN 13: 9780849335280
Author: Gary Howard, Matthew Kaser
Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols.
Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers-
- Commercially available adjuvants designed for the production of antisera in the research setting
- Methods for the production, purification, and characterization of antibodies
- Practical guidance to researchers needing to modify antibodies
- Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry
- Methods for applying immunolabeling to the realm of electron microscopy (EM)
- New antibody sources and new ways to use antobodies
Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.
Making and Using Antibodies A Practical Handbook 1st Edition Table of contents:
1. Introduction to Antibodies
- The Role of Antibodies in Research and Diagnostics
- Types of Antibodies: Polyclonal, Monoclonal, and Recombinant
- Key Concepts: Immunization, Antibody Binding, and Affinity
- Overview of Antibody Applications
2. Principles of Antibody Production
- Antigen Selection and Immunization Protocols
- Types of Immunogens and Their Selection
- Adjuvants: Role and Selection
- Ethical Considerations in Animal Use
3. Polyclonal Antibody Production
- Immunization of Animals for Polyclonal Antibody Production
- Serum Collection and Screening Methods
- Purification of Polyclonal Antibodies
- Determining Antibody Titer and Specificity
4. Monoclonal Antibody Production
- Hybridoma Technology: Principles and Methods
- Fusion of B Cells with Myeloma Cells
- Cloning, Screening, and Selection of Hybridomas
- Expansion and Maintenance of Hybridomas
5. Recombinant Antibodies
- Overview of Recombinant Antibody Technology
- Construction of Antibody Libraries
- Phage Display and Yeast Display Methods
- Cloning and Expression of Recombinant Antibodies
6. Antibody Purification
- General Strategies for Antibody Purification
- Protein A/G Chromatography
- Affinity Chromatography for Target-Specific Antibodies
- Ultrafiltration, Dialysis, and Ion Exchange Techniques
7. Labeling and Conjugation of Antibodies
- Labeling Antibodies with Fluorochromes, Enzymes, and Radioisotopes
- Biotinylation and Conjugation to Beads or Other Targets
- Strategies for Antibody Conjugation for Detection and Therapy
- Quality Control of Conjugated Antibodies
8. Antibody Characterization
- Methods for Assessing Antibody Specificity and Affinity
- Enzyme-Linked Immunosorbent Assay (ELISA)
- Western Blotting, Immunoprecipitation, and Flow Cytometry
- Determining Cross-reactivity and Epitope Mapping
9. Applications of Antibodies
- Antibodies in Immunohistochemistry and Immunofluorescence
- Flow Cytometry and Antibody Labeling
- Antibody Use in Protein Interaction Studies and Signaling Pathways
- Antibody Applications in Clinical Diagnostics and Therapy
10. Antibodies in Drug Development
- Therapeutic Monoclonal Antibodies: Production and Applications
- Antibodies as Immunotherapies in Cancer and Autoimmune Diseases
- Engineering Antibodies for Improved Therapeutic Efficacy
- Regulatory Aspects of Therapeutic Antibody Development
11. Handling and Storage of Antibodies
- Proper Storage Conditions for Antibodies
- Long-term Preservation of Antibody Stocks
- Handling Guidelines to Prevent Degradation and Contamination
- Antibody Shelf-life and Stability Testing
12. Troubleshooting in Antibody Use
- Common Problems in Antibody Production and Use
- Solutions for Low Antibody Yield or Specificity
- Antibody Cross-reactivity and Non-specific Binding
- Overcoming Challenges in Antibody Applications
13. Future Directions in Antibody Technology
- Advances in Antibody Engineering and Humanization
- Nanobodies and Single-Domain Antibodies
- Novel Antibody Formats: Bispecifics and Nanomaterials
- The Impact of Artificial Intelligence and High-throughput Screening
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