Basic DNA and RNA Protocols 1st edition by Adrian J Harwoo ISBN 089603402X 978-0896034020

Basic DNA and RNA Protocols 1st edition by Adrian J Harwoo – Ebook PDF Instant Download/Delivery. 089603402X, 978-0896034020
Full download Basic DNA and RNA Protocols 1st edition after payment

Product details:

ISBN 10: 089603402X
ISBN 13: 978-0896034020
Author: Adrian J Harwoo

An essential core collection of the latest molecular and genetic techniques for cloning, subcloning, sequencing, PCR, protein expression, and much more. Each protocol represents a time-tested, step-by-step recipe that creates an understanding of the procedure, easily reproducible results, and confidence that the procedure will work. The collection includes not only many updated and improved classic techniques, but also a powerful group of advanced methods that point to future progress, among them nonisotopic DNA labeling, silver staining, and automatic sequencing. This excellent bench companion will help those who need to learn for the first time how to conduct research on the molecular biology of nucleic acids or those who need to broaden their competence and laboratory skills. Even highly skilled researchers will find many time-saving techniques.

Basic DNA and RNA Protocols 1st Table of contents:

Preface:

• Introduction to Molecular Biology Protocols
• The Importance of DNA and RNA Techniques in Modern Research
• A Guide to Using This Book

Chapter 1: Fundamentals of DNA and RNA

• Structure and Function of DNA and RNA
• Differences Between DNA and RNA
• Key Terms and Concepts

Chapter 2: DNA Extraction Protocols

• Overview of DNA Extraction Methods
• Protocol 1: Standard DNA Extraction from Eukaryotic Cells
• Protocol 2: DNA Extraction from Bacterial Cells
• Troubleshooting DNA Extraction

Chapter 3: RNA Extraction Protocols

• Overview of RNA Extraction Methods
• Protocol 1: Total RNA Isolation from Eukaryotic Cells
• Protocol 2: RNA Isolation from Bacteria and Other Microorganisms
• Troubleshooting RNA Extraction

Chapter 4: Quantification and Purity Assessment

• Methods for Quantifying DNA and RNA
• Protocol 1: Spectrophotometric Analysis (A260/A280 Ratio)
• Protocol 2: Fluorometric Quantification of DNA and RNA
• Assessing Purity and Integrity of Nucleic Acids

Chapter 5: Polymerase Chain Reaction (PCR)

• The Basics of PCR: Amplifying DNA
• Protocol 1: Standard PCR Protocol
• Protocol 2: PCR Optimization for Specific Templates
• Troubleshooting Common PCR Problems

Chapter 6: Reverse Transcription and cDNA Synthesis

• The Role of Reverse Transcription in RNA Research
• Protocol 1: Reverse Transcription Reaction Setup
• Protocol 2: cDNA Synthesis from RNA Templates
• Troubleshooting Reverse Transcription

Chapter 7: Gel Electrophoresis of DNA and RNA

• Principles of Gel Electrophoresis
• Protocol 1: Agarose Gel Electrophoresis for DNA Analysis
• Protocol 2: Formaldehyde Gel Electrophoresis for RNA
• Visualizing DNA and RNA Bands: Staining Methods

Chapter 8: DNA Cloning and Vector Construction

• Introduction to DNA Cloning
• Protocol 1: Ligation of DNA Fragments into Vectors
• Protocol 2: Transformation of E. coli with Recombinant DNA
• Troubleshooting Cloning Procedures

Chapter 9: Northern and Southern Blotting

• The Basics of Blotting Techniques
• Protocol 1: Southern Blot for DNA Detection
• Protocol 2: Northern Blot for RNA Detection
• Troubleshooting Blotting Techniques

Chapter 10: RNA Analysis: Quantitative PCR and RT-PCR

• Introduction to Quantitative PCR for RNA Detection
• Protocol 1: Quantitative RT-PCR for Gene Expression Analysis
• Protocol 2: Relative Quantification and Data Normalization
• Troubleshooting Quantitative PCR

Chapter 11: DNA Sequencing

• Overview of DNA Sequencing Technologies
• Protocol 1: Sanger Sequencing: The Basics
• Protocol 2: Next-Generation Sequencing (NGS) Libraries Preparation
• Troubleshooting Sequencing Errors

Chapter 12: RNA Sequencing (RNA-Seq)

• Introduction to RNA-Seq Technology
• Protocol 1: RNA-Seq Library Construction
• Protocol 2: RNA-Seq Data Analysis
• Troubleshooting RNA-Seq Workflows

Chapter 13: DNA and RNA Storage and Handling

• Best Practices for Storing Nucleic Acids
• Protocol 1: Long-term Storage of DNA
• Protocol 2: RNA Storage and Preservation
• Minimizing Degradation During Handling

Chapter 14: Advanced Applications

• Techniques for DNA Methylation Analysis
• RNA Interference and Gene Silencing Protocols
• CRISPR-Cas9 for DNA Editing: Overview and Applications

Appendices:

• Appendix A: Common Troubleshooting Tips
• Appendix B: Troubleshooting PCR, RNA Extraction, and Sequencing
• Appendix C: Resources for Nucleic Acid Analysis

Index

People also search for Basic DNA and RNA Protocols 1st :

basic structure of dna and rna

basic differences between dna and rna

is dna and rna a protein

what are the dna and rna base pairing rules

dna and rna practice worksheet answers